Desensitization to Vasopressin Action in the Rat Kidney Medulla: Studies on Isolated Nephron Segments
| Autor: | P. Pradelles, I. Dublineau, C. De Rouffignac, Jean-Marc Elalouf |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Calcitonin
Male Vasopressin medicine.medical_specialty Urinary system medicine.medical_treatment Nephron In Vitro Techniques Biology Internal medicine Cyclic AMP medicine Renal medulla Animals Deamino Arginine Vasopressin Medulla Desensitization (medicine) Kidney Medulla Kidney Dose-Response Relationship Drug Rats Inbred Strains Nephrons General Medicine Glucagon Rats medicine.anatomical_structure Endocrinology Nephrology Cardiology and Cardiovascular Medicine hormones hormone substitutes and hormone antagonists |
| Zdroj: | Kidney and Blood Pressure Research. 15:57-65 |
| ISSN: | 1423-0143 1420-4096 |
| DOI: | 10.1159/000173442 |
| Popis: | Because of the prominent role of the renal medulla in the elaboration of concentrated urine and the possible differential regulation of the various nephron segments, desensitization to vasopressin (AVP) was studied on freshly isolated rat medullary thick ascending limbs (MTALs) and outer medullary collecting ducts (MCDs). Desensitization was induced through intramuscular injections of 1-deamino-8-D-arginine-vasopressin (dDAVP, 2 µg/day for 3 days), the last of which was performed 1 h before kidney removal. The cellular response to AVP was studied by measuring cAMP accumulation in micro-dissected nephron segments. In the MTAL, we observed a marked (around 80%) and selective desensitization to AVP, the response to either glucagon or human calcitonin remaining unaltered. In the MCD significant desensitization was observed in the presence of 1 nM AVP in the assay medium, and was no longer significant when the AVP concentration was increased to supramaximal levels (10-1,000nM). These experiments thus reveal a clear dissociation between MTAL and MCD with regards to dDAVP-induced desensitization and extend previous observations made on target segments in the renal cortex. |
| Databáze: | OpenAIRE |
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