Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize

Autor:	Jon P. Cody, Robert T. Gaeta, Morgan E. McCaw, Nathan C. Swyers, Changzeng Zhao, James A. Birchler, Nathaniel D. Graham
Rok vydání:	2019
Předmět:	Genetics Cloning chemistry.chemical_compound Transformation (genetics) chemistry Truncation fungi General Medicine Computational biology Biology DNA Telomere
Zdroj:	Current Protocols in Plant Biology
ISSN:	2379-8068
Popis:	Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required for the initiation of telomere-mediated truncation. The resulting DNA can then be used with truncation cassettes for introduction into maize via transformation. © 2016 by John Wiley & Sons, Inc. Keywords: minichromosomes; telomere-mediated truncation; telomere cloning
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33799cec11b3f163edd02e3ba7883db5 https://pubmed.ncbi.nlm.nih.gov/31725959 Zobrazit plný text záznamu Plný text