LEA polypeptide profiling of recalcitrant and orthodox legume seeds reveals ABI3-regulated LEA protein abundance linked to desiccation tolerance
| Autor: | Michaela Hundertmark, Julia Buitink, Julien Delahaie, Olivier Leprince, Hélène Rogniaux, Jérôme Bove |
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| Přispěvatelé: | Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), National Science Foundation (NSF) 703285, Region des Pays-de-la-Loire, France (QUALISEM 2009-2013), bilateral Partenariat Hubert Curien (PHC) program France-South Africa 25903RE, EU FP7 Marie Curie Individual Fellowship 252822, Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Proteome Physiology [SDV]Life Sciences [q-bio] heat stable proteome Plant Science 01 natural sciences Desiccation tolerance abscisic acid chemistry.chemical_compound Late embryogenesis abundant proteins Gene Expression Regulation Plant Electrophoresis Gel Two-Dimensional Abscisic acid proteomic dehydrin like protein Plant Proteins 0303 health sciences biology food and beverages medicago truncatula abi3 Adaptation Physiological Medicago truncatula Seeds recalcitrant seed Cotyledon Research Paper RNAseq food.ingredient desiccation tolerance Recalcitrant seed late embryogenesis abundant proteins rna seq 03 medical and health sciences proteomics food longevity Botany late embryogenesis abundant protein RNA Messenger Desiccation Castanospermum 030304 developmental biology stress tolerance maturation fungi arabidopsis thaliana Water Sequence Analysis DNA biology.organism_classification chemistry Mutation castanospermum australe gene expression medicago truncatula seed identification Peptides Transcriptome 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2013, 64 (14), pp.4559-4573. ⟨10.1093/jxb/ert274⟩ |
| ISSN: | 0022-0957 1460-2431 |
| DOI: | 10.1093/jxb/ert274⟩ |
| Popis: | In contrast to orthodox seeds that acquire desiccation tolerance during maturation, recalcitrant seeds are unable to survive drying. These desiccation-sensitive seeds constitute an interesting model for comparative analysis with phylogenetically close species that are desiccation tolerant.[br/]Considering the importance of LEA (late embryogenesis abundant) proteins as protective molecules both in drought and in desiccation tolerance, the heat-stable proteome was characterized in cotyledons of the legume Castanospermum australe and it was compared with that of the orthodox model legume Medicago truncatula. RNA sequencing identified transcripts of 16 homologues out of 17 LEA genes for which polypeptides are detected in M. truncatula seeds. It is shown that for 12 LEA genes, polypeptides were either absent or strongly reduced in C. australe cotyledons compared with M. truncatula seeds. Instead, osmotically responsive, non-seed-specific dehydrins accumulated to high levels in the recalcitrant cotyledons compared with orthodox seeds. Next, M. truncatula mutants of the ABSCISIC ACID INSENSITIVE3 (ABI3) gene were characterized. Mature Mtabi3 seeds were found to be desiccation sensitive when dried below a critical water content of 0.4g H2O g DW1. Characterization of the LEA proteome of the Mtabi3 seeds revealed a subset of LEA proteins with severely reduced abundance that were also found to be reduced or absent in C. australe cotyledons. Transcripts of these genes were indeed shown to be ABI3 responsive.[br/]The results highlight those LEA proteins that are critical to desiccation tolerance and suggest that comparable regulatory pathways responsible for their accumulation are missing in both desiccation-sensitive genotypes, revealing new insights into the mechanistic basis of the recalcitrant trait in seeds. |
| Databáze: | OpenAIRE |
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