Carbon nanotube amplification strategies for highly sensitive immunodetection of cancer biomarkers
| Autor: | Fotios Papadimitrakopoulos, Xin Yu, James F. Rusling, Sang N. Kim, Joseph D. Gong, J. Silvio Gutkind, Vyomesh Patel, Bernard Munge, Gary C. Jensen, John W. Gillespie, Ashwin Bhirde |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Enzyme-Linked Immunosorbent Assay Biochemistry Horseradish peroxidase Sensitivity and Specificity Catalysis Article Colloid and Surface Chemistry Antigen Prostate medicine Biomarkers Tumor Humans Detection limit Chromatography biology Chemistry Nanotubes Carbon General Chemistry Prostate-Specific Antigen Primary and secondary antibodies Prostate-specific antigen medicine.anatomical_structure biology.protein Immunohistochemistry Cancer biomarkers |
| Zdroj: | Journal of the American Chemical Society. 128(34) |
| ISSN: | 0002-7863 |
| Popis: | We describe herein the combination of electrochemical immunosensors using single-wall carbon nanotube (SWNT) forest platforms with multi-label secondary antibody-nanotube bioconjugates for highly sensitive detection of a cancer biomarker in serum and tissue lysates. Greatly amplified sensitivity was attained by using bioconjugates featuring horseradish peroxidase (HRP) labels and secondary antibodies (Ab(2)) linked to carbon nanotubes (CNT) at high HRP/Ab(2) ratio. This approach provided a detection limit of 4 pg mL(-)(1) (100 amol mL(-)(1)), for prostate specific antigen (PSA) in 10 microL of undiluted calf serum, a mass detection limit of 40 fg. Accurate detection of PSA in human serum samples was demonstrated by comparison to standard ELISA assays. PSA was quantitatively measured in prostate tissue samples for which PSA could not be differentiated by the gold standard immunohistochemical staining method. These easily fabricated SWNT immunosensors show excellent promise for clinical screening of cancer biomarkers and point-of-care diagnostics. |
| Databáze: | OpenAIRE |
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