Complete NMR assignment of a bisecting hybrid-type oligosaccharide transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase

Autor:	Yoshiki Oda, Takashi Yamanoi, Toshiyuki Inazu, Kenji Yamamoto, Kaname Katsuraya
Rok vydání:	2016
Předmět:	0301 basic medicine Glycosylation Magnetic Resonance Spectroscopy Stereochemistry Oligosaccharides Biochemistry Endo-M Substrate Specificity Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Bisecting hybrid-type oligosaccharide Mucor chemistry.chemical_classification biology Chemistry Organic Chemistry Glycoside General Medicine Nuclear magnetic resonance spectroscopy Oligosaccharide biology.organism_classification Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase 030104 developmental biology Mucor hiemalis Endo-β-N-acetylglucosaminidase Carbohydrate Sequence NMR spectral assignment Transglycosylation product Two-dimensional nuclear magnetic resonance spectroscopy Heteronuclear single quantum coherence spectroscopy
Zdroj:	Carbohydrate Research. 427:60-65
ISSN:	0008-6215
Popis:	This study describes the complete nuclear magnetic resonance (NMR) spectral assignment of a bisecting hybrid-type oligosaccharide 1, transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M). Through (1)H- and (13)C-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments, we determine the structure of the glycoside linkage formed by the Endo-M transglycosylation, i.e., the connection between GlcNAc and GlcNAc in oligosaccharide 1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3375aa3a8ddacb4db96f22c3ce3c1b0c https://doi.org/10.1016/j.carres.2016.03.013 Zobrazit plný text záznamu Full Text from ScienceDirect