Analysis of the 5'-upstream regions of the human relaxin H1 and H2 genes and their chromosomal localization on chromosome 9p24.1 by radiation hybrid and breakpoint mapping
| Autor: | K Csiszar, M Fox, S Povey, Gillian D. Bryant-Greenwood, JL Garibay-Tupas |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Fetal Membranes Premature Rupture TATA box Molecular Sequence Data Locus (genetics) Biology Hybrid Cells Response Elements Translocation Genetic Cell Line Endocrinology Pregnancy Coding region Humans Cloning Molecular Promoter Regions Genetic Molecular Biology Gene In Situ Hybridization Fluorescence Genetics Relaxin Base Sequence Breakpoint Physical Chromosome Mapping Chromosome Breakage TATA Box Multigene Family Female Chromosome breakage 5' Untranslated Regions Chromosomes Human Pair 9 |
| Zdroj: | Journal of molecular endocrinology. 23(3) |
| ISSN: | 0952-5041 |
| Popis: | Relaxins are known endocrine and autocrine/paracrine hormones that play a major role in reproduction. In the human there are two relaxin genes, H1 and H2 which share 90% sequence homology within their coding region. The biological and evolutionary significance of two highly homologous and biologically active human relaxins is unknown. In order to achieve a better understanding of the regulatory mechanisms involved in the differential expression of these two genes and to gain insight into their role(s) in the preterm premature rupture of the membranes, we have investigated the properties of their 5'-upstream regions and mapped them both by radiation hybrid and breakpoint mapping into the same chromosome 9p24.1 locus. The 5' ends of these relaxin genes could be divided into a proximal highly homologous segment and a distal non-homologous region. Within the proximal region are contained several putative regulatory elements common to both genes, suggesting a similar regulatory mechanism. The clustering of the relaxin genes within the same chromosomal locus suggests that these genes may be under a common regulation. On the other hand, a distinct gene-specific regulation may also exist for the individual relaxin genes since cis elements specific to each gene were identified at their 5' ends. Moreover, the observed divergence at the distal region of their 5'-upstream sequences may provide the structural features that act as gene-specific transcription regulators. Since the two genes are highly homologous in both their coding and flanking regions, the divergence at the distal region of their 5' ends may be important in the regulation of these genes and in their involvement in the pathology of preterm birth. |
| Databáze: | OpenAIRE |
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