Magnesium maintains the length of the circadian period in Arabidopsis
| Autor: | Didier Gonze, Christian Hermans, J. Romário F. de Melo, Alex A. R. Webb, Thomas De Caluwé, Annelie Gutsch, Nathalie Verbruggen, Jean-Christophe Leloup |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Time Factors Light Physiology Period (gene) Circadian clock Arabidopsis Endogeny Plant Science Cycloheximide 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Circadian Clocks Translational regulation Genetics Homeostasis Arabidopsis thaliana Magnesium Circadian rhythm Promoter Regions Genetic Research Articles 030304 developmental biology 0303 health sciences biology Arabidopsis Proteins Models Theoretical biology.organism_classification Circadian Rhythm Cell biology chemistry Seedlings Magnesium Deficiency Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Physiol |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | The circadian clock coordinates the physiological responses of a biological system to day and night rhythms through complex loops of transcriptional/translational regulation. It can respond to external stimuli and adjust generated circadian oscillations accordingly to maintain an endogenous period close to 24 h. However, the interaction between nutritional status and circadian rhythms in plants is poorly understood. Magnesium (Mg) is essential for numerous biological processes in plants, and its homeostasis is crucial to maintain optimal development and growth. Magnesium deficiency in young Arabidopsis thaliana seedlings increased the period of circadian oscillations of the CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) promoter (pCCA1:LUC) activity and dampened their amplitude under constant light in a dose-dependent manner. Although the circadian period increase caused by Mg deficiency was light dependent, it did not depend on active photosynthesis. Mathematical modeling of the Mg input into the circadian clock reproduced the experimental increase of the circadian period and suggested that Mg is likely to affect global transcription/translation levels rather than a single component of the circadian oscillator. Upon addition of a low dose of cycloheximide to perturb translation, the circadian period increased further under Mg deficiency, which was rescued when sufficient Mg was supplied, supporting the model’s prediction. These findings suggest that sufficient Mg supply is required to support proper timekeeping in plants. |
| Databáze: | OpenAIRE |
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