Solubility of folic acid and protonation of folate in NaCl at different concentrations, even in physiological solution
Autor: | Gaetano De Tommaso, Mauro Iuliano, Maria Rosa Festa, Antonietta D'Ambrosio, Emilio Bottari, Martina Meschino |
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Přispěvatelé: | Bottari, Emilio, D'Ambrosio, Antonietta, DE TOMMASO, Gaetano, Rosa Festa, Maria, Iuliano, Mauro, Meschino, Martina |
Rok vydání: | 2021 |
Předmět: |
Circular dichroism
Magnetic Resonance Spectroscopy 020209 energy Sodium Potentiometric titration Inorganic chemistry chemistry.chemical_element Protonation 02 engineering and technology Sodium Chloride Biochemistry Analytical Chemistry Absorbance Folic Acid 020401 chemical engineering 0202 electrical engineering electronic engineering information engineering Electrochemistry Environmental Chemistry 0204 chemical engineering Solubility Spectroscopy Chemistry Nuclear magnetic resonance spectroscopy Hydrogen-Ion Concentration Analytical Chemisty Equilibrium analysis Polymerization |
Zdroj: | The Analyst. 146:2339-2347 |
ISSN: | 1364-5528 0003-2654 |
Popis: | The solubility of folic acid was determined at 25 °C in 1.00 mol dm−3 and in 0.15 mol dm−3 NaCl (physiological solution) spectrophotometrically by measuring the absorbance of saturated solution at different hydrogen ion concentrations. Five protonation constants of folate were determined both from the dependence of the solubility on the hydrogen ion concentration as well as from potentiometric titrations carried out in the presence of solid folic acid and in alkaline solution, in which folate is relatively soluble. Corresponding to the protonation constants, nuclear magnetic resonance and florescence spectra were also obtained at different hydrogen ion concentrations to determine the protonation positions in acid, neutral and alkaline solutions. An approach through circular dichroism was also applied to study the eventual polymerization of folate in alkaline solution. |
Databáze: | OpenAIRE |
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