Antibody repertoire profiling with mimotope arrays
| Autor: | Stephan von Gunten, Anastas Pashov, Shina Pashova, Christoph Schneider |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pharmacology Phage display Microarray Mimotope Immunology Protein Array Analysis food and beverages High-Throughput Nucleotide Sequencing Computational biology Biology Bioinformatics Research Papers DNA sequencing Epitope Antibodies 03 medical and health sciences 030104 developmental biology Antibody Repertoire Peptide Library Immunology and Allergy Profiling (information science) Animals Humans DNA microarray |
| Zdroj: | Human vaccinesimmunotherapeutics. 13(2) |
| ISSN: | 2164-554X |
| Popis: | Large-scale profiling and monitoring of antibody repertoires is possible through next generation sequencing (NGS), phage display libraries and microarrays. These methods can be combined in a pipeline, which ultimately maps the antibody reactivities onto defined arrays of structures - peptides or carbohydrates. The arrays can help analyze the individual specificities or can be used as complex patterns. In any case, the targets recognized should formally be considered mimotopes unless they are proven to be epitopes driving the antibody synthesis. Here, the advantages and disadvantages of the major profiling techniques as well as their current and future application in disease prediction and vaccination are discussed. |
| Databáze: | OpenAIRE |
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