Serological diagnosis and prognostic of tegumentary and visceral leishmaniasis using a conserved Leishmania hypothetical protein
| Autor: | Rachel B. Caligiorne, Miguel A. Chávez-Fumagalli, Thaís T.O. Santos, Jamil S. Oliveira, Patrícia A.F. Ribeiro, Denise Utsch Gonçalves, Fernanda F. Ramos, Alexsandro Sobreira Galdino, Lourena E. Costa, Danielle F. de Magalhães-Soares, Daniel Dias, Áquila S.B. Portela, Mariana C. Duarte, Daniela P. Lage, Eduardo A.F. Coelho, Daniel Menezes-Souza, Beatriz C.S. Salles, Gerusa B. Carvalho, Eduardo Silva |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male 030231 tropical medicine Protozoan Proteins Leishmaniasis Cutaneous Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Leishmania braziliensis Serology 03 medical and health sciences Young Adult 0302 clinical medicine Dogs Antigen Medicine Animals Humans Chagas Disease Serologic Tests Amino Acid Sequence Dog Diseases Leishmania infantum biology business.industry Leishmaniasis Middle Aged medicine.disease biology.organism_classification Leishmania Prognosis Recombinant Proteins 030104 developmental biology Infectious Diseases Visceral leishmaniasis Antigens Helminth Immunology biology.protein Leishmaniasis Visceral Parasitology Female Antibody business Biomarkers |
| Zdroj: | Parasitology international. 67(3) |
| ISSN: | 1873-0329 |
| Popis: | New candidates for serological markers against leishmaniasis are required to be identified, since the presence of high titers of anti-Leishmania antibodies remain detected in sera of treated and cured patients, when current antigens have being employed. In this study, the diagnostic performance of a conserved Leishmania hypothetical protein was evaluated against a human and canine serological panel. The serological follow-up of the patients was also evaluated, using this recombinant antigen (rLiHyS) in ELISA assays. In the results, high sensitivity and specificity values were found when rLiHyS was used in the serological tests, while when the recombinant A2 (rA2) protein or an antigenic Leishmania preparation were used as controls, low sensitivity and specificity were found. Regarding the serological follow-up of the patients, significant reductions in the anti-rLiHyS antibody levels were found and, one year after the treatments, the anti-protein IgG production was similar to this found in the non-infected groups, reflecting a drop of the anti-rLiHyS antibody production. In conclusion, the present study shows for the first time a new recombinant antigen used to identify tegumentary and visceral leishmaniasis, as well as being able to serologically distinguish treated and cured patients from those developing active disease. |
| Databáze: | OpenAIRE |
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