3,3′-Dihydroxyisorenieratene prevents UV-induced formation of reactive oxygen species and the release of protein-bound zinc ions in human skin fibroblasts
| Autor: | Tanja Wagener, Silke De Spirt, Klaus-Dietrich Kröncke, Sebastian Kock, Wilhelm Stahl, Hans-Dieter Martin, Kaya Lutter |
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| Rok vydání: | 2009 |
| Předmět: |
Hot Temperature
Antioxidant Cell Survival Ultraviolet Rays medicine.medical_treatment chemistry.chemical_element Zinc Oxidative phosphorylation Quinolones Oxygen Cell Line Tosyl Compounds Stress Physiological medicine Humans Chelating Agents Fluorescent Dyes Skin chemistry.chemical_classification Reactive oxygen species Lutein Dose-Response Relationship Radiation Free Radical Scavengers Fibroblasts Carotenoids chemistry Biochemistry Photoprotection Reactive Oxygen Species Intracellular Food Science Biotechnology Cysteine |
| Zdroj: | Molecular Nutrition & Food Research. 54:285-291 |
| ISSN: | 1613-4125 |
| Popis: | 3,3'-Dihydroxyisorenieratene (DHIR) is a structurally unusual carotenoid exhibiting bifunctional antioxidant properties. It is synthesized by Brevibacterium linens, used in dairy industry for the production of red smear cheeses. The compound protects cellular structures against photo-oxidative damage and inhibits the UV-dependent formation of thymidine dimers. Here we show that DHIR prevents a UV-induced intracellular release of zinc ions from proteins in human dermal fibroblasts. The effect is correlated with a decreased formation of intracellular reactive oxygen species. In contrast, zinc release from cellular proteins induced by hyperthermia is not affected by pretreatment of cells with the antioxidant DHIR. It is suggested that the intracellular zinc release upon UV irradiation is due to oxidative modifications of the zinc ligands in proteins (e.g. cysteine) and that protection by DHIR is due to intracellular scavenging of reactive oxygen species generated in photo-oxidation. |
| Databáze: | OpenAIRE |
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