Analysis of antibody responses to selected Plasmodium falciparum merozoite surface antigens in mild and cerebral malaria and associations with clinical outcomes
| Autor: | Maguette Sylla Niang, Moustapha Mbow, Charlotte Joos, Birahim Niang, Marie-Louise Varela, Alioune Dieye, Moussa Fall, Cheikh Loucoubar, Babacar Mbengue, Ronald Perraut, Bécaye Fall |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cell Extracts
Male 0301 basic medicine Urban Population Antibodies Protozoan Subclass law.invention 0302 clinical medicine law Immunology and Allergy Medicine Malaria Falciparum Merozoite surface protein Child Merozoite Surface Protein 1 biology Middle Aged Recombinant Proteins Respiratory burst Hospitalization Treatment Outcome Cerebral Malaria Child Preschool Disease Progression Recombinant DNA Female Antibody Adult Adolescent Plasmodium falciparum Immunology Malaria Cerebral Antigens Protozoan Young Adult 03 medical and health sciences Antigen parasitic diseases Humans Aged Merozoites business.industry Infant Original Articles medicine.disease Survival Analysis 030104 developmental biology Immunoglobulin M biology.protein business Malaria 030215 immunology |
| Zdroj: | Clinical and Experimental Immunology. 196:86-96 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | Summary Merozoite surface proteins (MSPs) are critical for parasite invasion; they represent attractive targets for antibody-based protection against clinical malaria. To identify protection-associated target MSPs, the present study analysed antibody responses to whole merozoite extract (ME) and to defined MSP recombinant antigens in hospitalized patients from a low endemic urban area as a function of disease severity (mild versus cerebral malaria). Sera from 110 patients with confirmed severe cerebral malaria (CM) and 91 patients with mild malaria (MM) were analysed (mean age = 29 years) for total and subclass immunoglobulin (Ig)G to ME and total IgG to MSP1p19, MSP2, MSP3, MSP4 and MSP5 by enzyme-linked immunosorbent assay (ELISA). Functional antibody responses were evaluated using the antibody-dependent respiratory burst (ADRB) assay in a subset of sera. There was a trend towards higher IgG1 and IgG4 levels to ME in CM compared to MM; only ME IgM responses differed significantly between fatal and surviving CM patients. Increased prevalence of IgG to individual MSPs was found in the CM compared to the MM group, including significantly higher levels of IgG to MSP4 and MSP5 in the former. Sera from fatal (24·5%) versus surviving cases showed significantly lower IgG to MSP1p19 and MSP3 (P < 0·05). ADRB assay readouts correlated with high levels of anti-MSP IgG, and trended higher in sera from patients with surviving compared to fatal CM outcome (P = 0·07). These results document strong differential antibody responses to MSP antigens as targets of protective immunity against CM and in particular MSP1p19 and MSP3 as prognostic indicators. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |