Chimeric mice reveal clonal development of pancreatic acini, but not islets
| Autor: | Timothy Nottoli, Neil D. Theise, E. Scott Swenson, Julie Xanthopoulos, James L. McGrath, Diane S. Krause |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Cell Crypt Biophysics Biology Biochemistry Article Chimera (genetics) Islets of Langerhans Mice Cell Movement Internal medicine Y Chromosome medicine Animals Progenitor cell Molecular Biology Embryonic Stem Cells In Situ Hybridization Fluorescence Chimera Pancreatic islets Cell Biology Embryonic stem cell Pancreas Exocrine Cell biology Clone Cells Intestines Mice Inbred C57BL medicine.anatomical_structure Endocrinology Female Stem cell Pancreas |
| Zdroj: | Biochemical and biophysical research communications. 379(2) |
| ISSN: | 1090-2104 |
| Popis: | Intestinal crypt stem cells establish clonal descendants. To determine whether the pancreas is patterned by a similar process, we used embryonic stem (ES) cell chimeric mice, in which male ES cells were injected into female blastocysts. Fluorescence in situ hybridization for the Y chromosome (Y-FISH) revealed clonal patterning of ES-derived cells in the adult mouse small intestine and pancreas. Intestinal crypts were entirely male or entirely female. Villi contained columns of male or female epithelial cells, consistent with upward migration of cells from the crypts which surround them. Within the exocrine pancreas, acini were entirely male or entirely female, consistent with patterning from a single stem/progenitor cell. Pancreatic islets contained a mixture of male and female cells, consistent with patterning from multiple progenitors. Male-female chimeric mice demonstrate that the adult mouse exocrine pancreatic acinus is patterned from a single stem/progenitor cell, while the endocrine pancreas arises from multiple progenitors. |
| Databáze: | OpenAIRE |
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