Extreme calorie restriction in yeast retentostats induces uniform non-quiescent growth arrest
| Autor: | Pascale Daran-Lapujade, Jack T. Pronk, Anne Doerr, Frank J. Bruggeman, Markus M.M. Bisschops, Peter J.T. Verheijen, Marijke A. H. Luttik |
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| Přispěvatelé: | Systems Bioinformatics, AIMMS, Mathematics |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins 030106 microbiology Calorie restriction Saccharomyces cerevisiae 03 medical and health sciences Bioreactors Growth arrest Gene Expression Regulation Fungal Molecular Biology Heat-Shock Proteins Extreme calorie restriction biology Experimental model Cell Biology Cell Cycle Checkpoints Retentostat biology.organism_classification Yeast Actins Culture Media 030104 developmental biology Glucose Biochemistry Homogeneous Batch Cell Culture Techniques Actin structure Non-dividing Heterogeneity mRNA FISH Metabolic activity Energy Metabolism |
| Zdroj: | Biochimica et Biophysica Acta-Molecular Cell Research, 1864(1), 231-242. Elsevier Bisschops, M M M, Luttik, M A H, Doerr, A, Verheijen, P J T, Bruggeman, F, Pronk, J T & Daran-Lapujade, P 2017, ' Extreme calorie restriction in yeast retentostats induces uniform non-quiescent growth arrest ', Biochimica et Biophysica Acta-Molecular Cell Research, vol. 1864, no. 1, pp. 231-242 . https://doi.org/10.1016/j.bbamcr.2016.11.002 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/j.bbamcr.2016.11.002 |
| Popis: | Non-dividing Saccharomyces cerevisiae cultures are highly relevant for fundamental and applied studies. However, cultivation conditions in which non-dividing cells retain substantial metabolic activity are lacking. Unlike stationary-phase (SP) batch cultures, the current experimental paradigm for non-dividing yeast cultures, cultivation under extreme calorie restriction (ECR) in retentostat enables non-dividing yeast cells to retain substantial metabolic activity and to prevent rapid cellular deterioration. Distribution of F-actin structures and single-cell copy numbers of specific transcripts revealed that cultivation under ECR yields highly homogeneous cultures, in contrast to SP cultures that differentiate into quiescent and non-quiescent subpopulations. Combined with previous physiological studies, these results indicate that yeast cells subjected to ECR survive in an extended G1 phase. This study demonstrates that yeast cells exposed to ECR differ from carbon-starved cells and offer a promising experimental model for studying non-dividing, metabolically active, and robust eukaryotic cells. |
| Databáze: | OpenAIRE |
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