Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa
| Autor: | Fabien Nogué, Lionel Gissot, Céline Morineau, Frédérique Tellier, Zsolt Kelemen, Yannick Bellec, Jean-Denis Faure |
|---|---|
| Přispěvatelé: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Carnot 3BCar program CamelinOil2 (contract 75000058), Agence National de la Recherche (program ANR-11-BTBR-0001-GENIUS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
FAD2
Fatty Acid Desaturases 0106 biological sciences 0301 basic medicine IJPB\₂016a-ACL brassicaceae [SDV]Life Sciences [q-bio] Camelina sativa Mutant Gene Dosage Mutagenesis (molecular biology technique) Plant Science 01 natural sciences Genome Gene dosage Camelina 03 medical and health sciences CRISPR-Cas9 oleic acid Genetic variability Gene Research Articles Plant Proteins Gene Editing 2. Zero hunger Genetics biology camelina sativa food and beverages 15. Life on land Plants Genetically Modified biology.organism_classification hexaploïde phénotype 030104 developmental biology graine oléagineuse CRISPR-Cas Systems CRISPR‐Cas9 Agronomy and Crop Science Research Article amélioration des plantes 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Biotechnology Journal Plant Biotechnology Journal, 2017, 15 (6), pp.729-739. ⟨10.1111/pbi.12671⟩ Plant Biotechnology Journal 6 (15), 729-739. (2017) |
| DOI: | 10.1111/pbi.12671⟩ |
| Popis: | International audience; In many plant species, gene dosage is an important cause of phenotype variation. Selectively engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely-related expressed sub-genomes, is an ideal species for investigation of the possibility of creating a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta-12-desaturase (FAD2) genes was achieved by CRISPR-Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil. Analysis of mutations over four generations demonstrated the presence of a large variety of heritable mutations in the three isologous CsFAD2 genes. The different combinations of single, double and triple mutants in the T3 generation were isolated, and the complete loss-of-function mutants revealed the importance of delta-12-desaturation for Camelina development. Combinatorial association of different alleles for the three FAD2 loci provided a large diversity of Camelina lines with various lipid profiles, ranging from 10 to 62% oleic acid accumulation in the oil. The different allelic combinations allowed an unbiased analysis of gene dosage and function in this hexaploid species, but also provided a unique source of genetic variability for plant breeding. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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