Fungi allergens produced by solid-state fermentation process: optimization and allergen characterization
| Autor: | Maria Helena Andrade Santana, Ricardo de Lima Zollner, Walderez Gambale, Salah D. M. Hasan |
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| Rok vydání: | 2003 |
| Předmět: |
Biomass
Bioengineering Applied Microbiology and Biotechnology Biochemistry Helminthosporium Botany Confidence Intervals Protein precipitation Food science Molecular Biology Bran biology Chemistry food and beverages Substrate (chemistry) General Medicine Allergens Hydrogen-Ion Concentration biology.organism_classification Kinetics Solid-state fermentation Polyphenol Fermentation Drechslera Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. |
| ISSN: | 0273-2289 |
| Popis: | Allergenic extracts were produced from Drechslera (Helminthosporium) monoceras biomass cultured by solid-state fermentation using wheat bran as the substrate. The main fermentation variables were selected by statistical design, and the optimized biomass yield (1.43 mg/[g of dry substrate d]) was obtained at pH 9.5 and 45.8% moisture. The allergenic extracts were produced from crude extract by protein precipitation and polyphenol removal. Proteins in the range of 16-160 kDa were identified in the extracts. Their reactions in patients were characterized by in vivo cutaneous tests (positive in 40% of the atopic patients) and by dot-blotting assays. |
| Databáze: | OpenAIRE |
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