3D resolution enhancement of deep-tissue imaging based on virtual spatial overlap modulation microscopy
| Autor: | Kuo-Jen Hsu, I-Cheng Su, Yen-Yin Lin, Shi-Wei Chu, Po-Ting Shen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Blind deconvolution Diffraction Laser Scanning Microscopy Laser scanning business.industry Computer science Resolution (electron density) 01 natural sciences Atomic and Molecular Physics and Optics law.invention 010309 optics 03 medical and health sciences 030104 developmental biology Optics Optical imaging Optical microscope Modulation law 0103 physical sciences Microscopy Near-field scanning optical microscope Deconvolution business |
| Zdroj: | Optics express. 24(15) |
| ISSN: | 1094-4087 |
| Popis: | During the last decades, several resolution enhancement methods for optical microscopy beyond diffraction limit have been developed. Nevertheless, those hardware-based techniques typically require strong illumination, and fail to improve resolution in deep tissue. Here we develop a high-speed computational approach, three-dimensional virtual spatial overlap modulation microscopy (3D-vSPOM), which immediately solves the strong-illumination issue. By amplifying only the spatial frequency component corresponding to the un-scattered point-spread-function at focus, plus 3D nonlinear value selection, 3D-vSPOM shows significant resolution enhancement in deep tissue. Since no iteration is required, 3D-vSPOM is much faster than iterative deconvolution. Compared to non-iterative deconvolution, 3D-vSPOM does not need a priori information of point-spread-function at deep tissue, and provides much better resolution enhancement plus greatly improved noise-immune response. This method is ready to be amalgamated with two-photon microscopy or other laser scanning microscopy to enhance deep-tissue resolution. |
| Databáze: | OpenAIRE |
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