Epidemiology and susceptibilities to mercury preservatives of staphylococci isolated from used eye-drops preserved with thiomersal
| Autor: | S K Du Bois, A L Davison, R. J. Pinney |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Preservative
Micrococcaceae medicine.drug_class Drug Storage Staphylococcus Pharmaceutical Science Microbial Sensitivity Tests medicine.disease_cause Dexamethasone Microbiology Minimum inhibitory concentration chemistry.chemical_compound Antiseptic Staphylococcus hominis medicine Humans Pharmacology biology Thimerosal Preservatives Pharmaceutical Drug Resistance Microbial biology.organism_classification Phenylmercury Compounds chemistry Mercuric Chloride Anti-Infective Agents Local Electrophoresis Polyacrylamide Gel Thiomersal Ophthalmic Solutions Bacteria Plasmids |
| Zdroj: | The Journal of pharmacy and pharmacology. 47(3) |
| ISSN: | 0022-3573 |
| Popis: | Minimum inhibitory concentrations (MICs) of seven independent isolates of Staphylococcus hominis isolated in the same week from used eye-drops, preserved with thiomersal and collected from wards and clinics in the same hospital, ranged between 1 and 0·03 mg L−1 for thiomersal, 1 and 0·01 mg L−1 for phenyl mercuric nitrate and 10 and 3 mg L−1 for mercuric chloride. Although MIC values determined on solid nutrient medium indicated a 100-fold variation in susceptibility to the bacteriostatic effect of phenyl mercuric nitrate, after 5 h in an aqueous solution containing the bactericidal concentration of 10 mg L−1 phenyl mercuric nitrate, the survival levels of the six S. hominis isolates were similar, with a mean of 13·4% (s.d. 11·0), compared with 100 and 0·8%, respectively, for the most resistant and most sensitive control staphylococcal strains tested. Antibiotic susceptibilities and plasmid profiles of the S. hominis isolates indicated they were the same strain. It is concluded that laboratory indicators of preservative efficacy, such as MIC determination or susceptibility to bactericidal concentrations of preservatives, do not necessarily correlate with the epidemiology of contaminating bacterial strains or their survival in preserved pharmaceuticals. |
| Databáze: | OpenAIRE |
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