CRISPR‐Cas enzymes: The toolkit revolutionizing diagnostics
| Autor: | Elizabeth M Hawkins, Janice S. Chen, Bridget McKay, Lior Kreindler, Emel Alpay, Shae A Hubbell, Matthew S. Verosloff, Deepika Verma, James P Broughton, Carley G Hendriks, Sonal Jain, Emma G Stanfield, Sarah Jane Shapiro, Benjamin A Blizard |
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| Rok vydání: | 2021 |
| Předmět: |
Gene Editing
2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) Global challenges Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) COVID-19 General Medicine Computational biology Endonucleases Applied Microbiology and Biotechnology Humans Molecular Medicine CRISPR CRISPR-Cas Systems Pandemics Nucleic acid detection |
| Zdroj: | Biotechnology Journal. 17:2100304 |
| ISSN: | 1860-7314 1860-6768 |
| Popis: | The programmable nature of sequence-specific targeting by CRISPR-Cas nucleases has revolutionized a wide range of genomic applications and is now emerging as a method for nucleic acid detection. We explore how the diversity of CRISPR systems and their fundamental mechanisms have given rise to a wave of new methods for target recognition and readout. These cross-disciplinary advances found at the intersection of CRISPR biology and engineering have led to the ability to rapidly generate solutions for emerging global challenges like the COVID-19 pandemic. We further discuss the advances and potential for CRISPR-based detection to have an impact across a continuum of diagnostic applications. |
| Databáze: | OpenAIRE |
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