Stage and tissue expression patterns of Schistosoma mansoni venom allergen-like proteins SmVAL 4, 13, 16 and 24
| Autor: | Luciana C. C. Leite, Leonardo P. Farias, Mayra M.F. Barbosa, Patricia A. Miyasato, Tereza Cristina Barbosa, Eliana Nakano, R.S. Fernandes |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030231 tropical medicine Snails Short Report Gene Expression Venom In situ hybridization WISH Biology lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Immune system Downregulation and upregulation Gene expression Animals Humans lcsh:RC109-216 Cercaria Gene In Situ Hybridization Germ ball Life Cycle Stages Venoms Schistosoma mansoni Allergens biology.organism_classification SmVAL In vitro Acetabular glands Acetabularia Cell biology Up-Regulation 030104 developmental biology Infectious Diseases Antigens Helminth Immunology Host-Pathogen Interactions Parasitology Oesophageal glands |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 10, Iss 1, Pp 1-13 (2017) |
| ISSN: | 1756-3305 |
| Popis: | Background Schistosoma mansoni venom allergen-like protein (SmVAL) is a gene family composed of 29 members divided into group 1 encoding proteins potentially secreted, and group 2 encoding intracellular components. Some members were found to be upregulated in the transition of germ ball - cercariae - day 3 schistosomula, suggesting that group 1 SmVAL proteins are associated with the invasion of the human host, although their functions are not completely established. Recently, we have described the localization of SmVAL7 (group 1) and SmVAL6 (group 2) transcripts in the oesophageal gland and in the oral and ventral suckers of adult parasites, respectively. The expression patterns of the two genes suggest that SmVAL7 protein plays a role in the blood-feeding process while SmVAL6 is associated with the parasite attachment and movement in the vasculature. In this way, searching for additional secreted SmVAL proteins that could be involved in key processes from skin penetration to the beginning of blood-feeding, we investigated the tissue localization of SmVAL4, 13, 16 and 24 by whole-mount in situ hybridization (WISH). Results We report here the localization of group 1 SmVAL4 and 24 transcripts in the pre-acetabular glands of developing germ balls. Time course experiments of in vitro cultured schistosomula after cercariae transformation demonstrated that SmVAL4 protein is secreted during the first 3 h of in vitro culture, correlating with the emptying of acetabular glands as documented by confocal microscopy. In addition, the localization of SmVAL13 transcripts in adult male anterior oesophageal gland suggests that the respective protein may be involved in the first steps of the blood-feeding process. SmVAL16 was localized close to the neural ganglia and requires further investigation. Conclusions Our findings demonstrate that SmVAL proteins have localizations that place them in strategic positions to be considered as potential vaccine candidates as some members are exposed to interaction with the immune system and may participate in key processes of mammalian invasion and parasitism establishment. Electronic supplementary material The online version of this article (doi:10.1186/s13071-017-2144-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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