Evaluation of Interleukin-4 Concentration by ELISA Is Influenced by the Consumption of IL-4 by Cultured Cells
| Autor: | Maria E. Baca-Estrada, Catherine Ewen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Th2 response
animal diseases Immunology Exotoxins Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Context (language use) Biology Lymphocyte Activation Binding Competitive Epitopes Interferon-gamma Mice Immune system Virology Animals Cells Cultured Interleukin 4 Immune mechanisms Mice Inbred BALB C Immune Sera Cell Biology biochemical phenomena metabolism and nutrition Receptors Interleukin-4 Mice Inbred C57BL bacteria Female Cytokine secretion Binding Sites Antibody Interleukin-4 Spleen |
| Zdroj: | Journal of Interferon & Cytokine Research. 21:39-43 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/107999001459141 |
| Popis: | The ability to accurately measure cytokine secretion by immune cells is critical to the evaluation of immune mechanisms in the context of Th1 and Th2 responses. In this study, we demonstrated that in vitro consumption of interleukin-4 (IL-4) by stimulated cells influences the concentration of IL-4 in the culture supernatant. In contrast, evaluation of IL-4-secreting cells by ELISPOT is not influenced by the consumption of IL-4 by cultured cells. These discrepancies influence the cytokine profile when responses are evaluated in relation to the secretion of other cytokines, for example, interferon-gamma (IFN-gamma). This information ultimately should enable investigators to evaluate immune responses accurately without concerns of bias resulting from in vitro consumption of IL-4, thus providing much more reliable interpretations of the type of immune response being evaluated. |
| Databáze: | OpenAIRE |
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