| Popis: |
(A) The phosphorylation of Akt was blocked by the inhibitors of IGF-1R and PI3K. MCF-7:5C cells were treated with vehicle (0.1% DMSO; con), E2 (10-9 mol/L), AG1024 (5×10-6 mol/L), E2 (10-9 mol/L) plus AG1024 (5×10-6 mol/L), LY294002 (10-6 mol/L), and E2 (10-9 mol/L) plus LY294002 (10-6 mol/L) for 48 hours. p-Akt was examined by Western blot. Total Akt was measured as loading control. (B) Effects of the c-Src inhibitor on the expression of Akt. MCF-7:5C cells were treated with vehicle (0.1% DMSO; con), E2 (10-9 mol/L), PP2 (5×10-6 mol/L), and E2 (10-9 mol/L) plus PP2 (5×10-6 mol/L) for different time points indicated. p-Akt and total-Akt were examined by Western blot. β-actin was measured as loading control. |
