Development of a universal, tunable, miniature fluorescence microscope for use at the point of care
| Autor: | Tomasz S. Tkaczyk, Cynthia Wong, Michal E. Pawlowski, Catherine E. Majors, Rebecca Richards-Kortum, Alessandra Forcucci |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Single use business.industry Acridine orange Image processing 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence Article Atomic and Molecular Physics and Optics 010309 optics chemistry.chemical_compound chemistry 0103 physical sciences Fluorescence microscope Optoelectronics 0210 nano-technology business Focus (optics) Biotechnology Point of care |
| Zdroj: | Biomedical Optics Express. 9:1041 |
| ISSN: | 2156-7085 |
| Popis: | Fluorescence microscopy can be a powerful tool for cell-based diagnostic assays; however, imaging can be time consuming and labor intensive to perform. Tunable systems give the ability to electronically focus at user selected depths inside an object volume and may simplify the opto-mechanical design of the imaging system. We present a prototype of a universal, tunable, miniature fluorescence microscope built from poly(methyl methacrylate) singlets that incorporates miniature, electrowetted lenses for electronic focusing. We demonstrate the ability of this system to perform clinically relevant differential white blood cell counts using single use custom cartridges pre-loaded with the fluorescent dye acridine orange. |
| Databáze: | OpenAIRE |
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