Alterations in membrane type-1 matrix metalloproteinase abundance after the induction of thoracic aortic aneurysm in a murine model

Autor: Jeffrey A. Jones, Jean Marie Ruddy, John S. Ikonomidis, Theresa A. Brinsa, Shenikqua Bouges, Francis G. Spinale, Rupak Mukherjee, Robert E. Stroud, Juozas A. Zavadzkas
Rok vydání: 2010
Předmět:
Male
Pathology
medicine.medical_specialty
Time Factors
Physiology
Blotting
Western
Contrast Media
Aorta
Thoracic
Biology
Matrix metalloproteinase
Thoracic aortic aneurysm
Gene Expression Regulation
Enzymologic
Extracellular matrix
Calcium Chloride
Mice
Aortic aneurysm
Downregulation and upregulation
Physiology (medical)
medicine.artery
Matrix Metalloproteinase 14
medicine
Animals
Thoracic aorta
RNA
Messenger
Fibroblast
Ultrasonography
Tissue Inhibitor of Metalloproteinase-2
Microbubbles
Aortic Aneurysm
Thoracic
Reverse Transcriptase Polymerase Chain Reaction
Articles
Fibroblasts
medicine.disease
Immunohistochemistry
Up-Regulation
Mice
Inbred C57BL
Disease Models
Animal
medicine.anatomical_structure
Circulatory system
Matrix Metalloproteinase 2
Female
Cardiology and Cardiovascular Medicine
Dilatation
Pathologic
Zdroj: American Journal of Physiology-Heart and Circulatory Physiology. 299:H114-H124
ISSN: 1522-1539
0363-6135
Popis: Thoracic aortic aneurysms (TAAs) develop as a result of dysregulated extracellular matrix remodeling mediated by several matrix metalloproteinases (MMPs). Membrane type-1 MMP (MT1-MMP) is the prototypical member of a unique family of membrane-bound MMPs, possessing multiple substrates and functions. The present study tested the hypothesis that MT1-MMP expression, abundance, and activity would be elevated during TAA development and that this protease is produced primarily by mesenchymal cells within the thoracic aorta. Descending thoracic aortas were harvested from C57BL/6J mice at multiple time points (2, 4, 8, and 16 wk, n = 15 each) post-TAA induction (0.5M CaCl2, 15 min) and compared with reference controls ( n = 15). The expression and abundance of MT1-MMP, MMP-2, and tissue inhibitor of metalloproteinase (TIMP)-2 were assessed by quantitative PCR and immunoblot analysis. MT1-MMP activity was determined by fluorescent peptide assay. MT1-MMP was localized within the aortic wall by immunohistochemistry. MT1-MMP abundance and localization in live animals (8 wk post-TAA induction vs. control) was determined by microultrasound imaging with an MT1-MMP-targeted microbubble contrast agent. Aortic diameter was increased 172 ± 7% at 16 wk post-TAA induction ( P < 0.05). MT1-MMP and MMP-2 mRNA levels were elevated at 2 wk post-TAA induction ( P < 0.05). MT1-MMP protein abundance increased progressively to a maximum of 178 ± 26% at 16 wk post-TAA induction, whereas MMP-2 and TIMP-2 peaked at 2 wk post-TAA induction (526 ± 93% and 376 ± 48%, respectively, P < 0.05). MT1-MMP colocalized with fibroblasts, and MT1-MMP-targeted contrast binding was elevated in 8-wk TAA-induced mice versus control mice (217 ± 53% vs. 81 ± 8%, P < 0.05). In conclusion, these novel results suggest that MT1-MMP plays a dynamic multifunctional role in TAA development and, therefore, may provide a significant target for therapeutic strategies.
Databáze: OpenAIRE
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