A 3D polydimethylsiloxane microhourglass-shaped channel array made by reflowing photoresist structures for engineering a blood capillary network
| Autor: | Henk-Willem Veltkamp, Han Gardeniers, Séverine Le Gac, Pamela Habibovic, Danielle Baptista, Hoon Suk Rho |
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| Přispěvatelé: | Mesoscale Chemical Systems, MESA+ Institute, Integrated Devices and Systems, TechMed Centre, Applied Microfluidics for BioEngineering Research, Division Instructive Biomaterials Eng, RS: MERLN - Instructive Biomaterials Engineering (IBE) |
| Rok vydání: | 2020 |
| Předmět: |
Lithography
Fabrication Materials science Channel (digital image) Thermal reflow of positive photoresist UT-Hybrid-D Nanotechnology Photoresist General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences chemistry.chemical_compound law Humans Dimethylpolysiloxanes Artificial capillary network Molecular Biology Microscale chemistry 030304 developmental biology 0303 health sciences Microchannel Polydimethylsiloxane 030302 biochemistry & molecular biology Endothelial Cells chemistry Three dimensional (3D) hourglass-shaped microstructures Photolithography |
| Zdroj: | Methods, 190, 63-71. Academic Press Inc. Methods, 190, 63-71. Elsevier Science |
| ISSN: | 1046-2023 |
| Popis: | This paper describes an innovative yet straightforward fabrication technique to create three-dimensional microstructures with controllable tapered geometries by combining conventional photolithography and thermal reflow of photoresist. Positive photoresist-based microchannel structures with varying width-to-length ratios were reflowed after their fabrication to generate three-dimensional funnel structures with varying curvatures. A polydimethylsiloxane hourglass-shaped microchannel array was next cast on these photoresist structures, and primary human lung microvascular endothelial cells were cultured in the device to engineer an artificial capillary network. Our work demonstrates that this cost-effective and straightforward fabrication technique has great potential in engineering three-dimensional microstructures for biomedical and biotechnological applications such as blood vessel regeneration strategies, drug screening for vascular diseases, microcolumns for bioseparation, and other fluid dynamic studies at microscale. |
| Databáze: | OpenAIRE |
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