Juniperus indica Bertol. extract synergized with cisplatin against melanoma cells via the suppression of AKT/mTOR and MAPK signaling and induction of cell apoptosis
Autor: | Xiao-Fan Huang, Hong-Wei Gao, Kai-Fu Chang, Li-Ting Tang, Shan-Chih Lee, Nu-Man Tsai |
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Rok vydání: | 2021 |
Předmět: |
Skin Neoplasms
Cell cycle checkpoint Juniperus indica Bertol MAP Kinase Signaling System Apoptosis Madin Darby Canine Kidney Cells anti-proliferation Mice 03 medical and health sciences Dogs 0302 clinical medicine Cyclin-dependent kinase synergism Cell Line Tumor Antineoplastic Combined Chemotherapy Protocols medicine Animals Humans Melanoma Protein kinase B Caspase PI3K/AKT/mTOR pathway Cell Proliferation Cisplatin biology Plant Extracts Chemistry Cell growth TOR Serine-Threonine Kinases Drug Synergism General Medicine G1 Phase Cell Cycle Checkpoints Juniperus biology.protein Cancer research 030211 gastroenterology & hepatology Proto-Oncogene Proteins c-akt Research Paper Signal Transduction medicine.drug |
Zdroj: | International Journal of Medical Sciences |
ISSN: | 1449-1907 |
DOI: | 10.7150/ijms.49423 |
Popis: | Juniperus indica Bertol. is an herbal plant that belongs to the genus Juniperus, which is commonly used in traditional medicine to refresh the mind and for diuretic use. However, few studies have reported the function of J. indica Bertol. Hence, this study aimed to investigate the anti-tumor and synergistic potential of J. indica Bertol. extract (JIB extract) for melanoma cells. Our results indicated the anti-melanoma activity of JIB extract. JIB extract induced cell cycle arrest at the G0/G1 phase and decreased cyclin and cdk protein expressions. In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. According to the JIB extract's anti-melanoma capacity, to assess the synergistic effects of cisplatin and JIB extract. The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. |
Databáze: | OpenAIRE |
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