Biomimetic human small muscular pulmonary arteries
| Autor: | Frederic B. Askin, Annie Hou, Hoku West-Foyle, Qianru Jin, Scot C. Kuo, Jayson V. Pagaduan, Dan E. Berkowitz, Anil Bhatta, Thao D. Nguyen, Lewis H. Romer, David H. Gracias, Jiayu Liu, Xing Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Spatial positioning
Myocytes Smooth Muscle 02 engineering and technology Pulmonary Artery Biology Nitric oxide Extracellular matrix 03 medical and health sciences chemistry.chemical_compound Human disease Smooth muscle Biomimetics medicine Humans Health and Medicine Research Articles Cells Cultured Mechanical Phenomena 030304 developmental biology 0303 health sciences Multidisciplinary Tissue Engineering SciAdv r-articles Muscle Smooth Models Theoretical 021001 nanoscience & nanotechnology medicine.disease Pulmonary hypertension Coculture Techniques 3. Good health Structure and function Applied Sciences and Engineering chemistry 0210 nano-technology Neuroscience Algorithms Biomarkers Research Article Signal Transduction Biofabrication |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| Popis: | Biofabricated mass-producible arteries containing layered and aligned cells and matrix exhibit enhanced cellular function. Changes in structure and function of small muscular arteries play a major role in the pathophysiology of pulmonary hypertension, a burgeoning public health challenge. Improved anatomically mimetic in vitro models of these microvessels are urgently needed because nonhuman vessels and previous models do not accurately recapitulate the microenvironment and architecture of the human microvascular wall. Here, we describe parallel biofabrication of photopatterned self-rolled biomimetic pulmonary arterial microvessels of tunable size and infrastructure. These microvessels feature anatomically accurate layering and patterning of aligned human smooth muscle cells, extracellular matrix, and endothelial cells and exhibit notable increases in endothelial longevity and nitric oxide production. Computational image processing yielded high-resolution 3D perspectives of cells and proteins. Our studies provide a new paradigm for engineering multicellular tissues with precise 3D spatial positioning of multiple constituents in planar moieties, providing a biomimetic platform for investigation of microvascular pathobiology in human disease. |
| Databáze: | OpenAIRE |
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