| Autor: |
Shidan Tosif, Ebene R. Haycroft, Sohinee Sarkar, Zheng Quan Toh, Lien Anh Ha Do, Celeste M. Donato, Kevin J. Selva, Monsurul Hoq, Isabella Overmars, Jill Nguyen, Lai‐Yang Lee, Vanessa Clifford, Andrew Daley, Francesa L. Mordant, Jodie McVernon, Kim Mulholland, Adrian J. Marcato, Miranda Z. Smith, Nigel Curtis, Sarah McNab, Richard Saffery, Katherine Kedzierska, Kanta Subarrao, David Burgner, Andrew Steer, Julie E. Bines, Philip Sutton, Paul V. Licciardi, Amy W. Chung, Melanie R. Neeland, Nigel W. Crawford |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology. 33(7) |
| ISSN: |
1399-3038 |
| Popis: |
Background Household studies are crucial for understanding the transmission of SARS-CoV-2 infection, which may be underestimated from PCR testing of respiratory samples alone. We aim to combine assessment of household mitigation measures; nasopharyngeal, saliva and stool PCR testing; along with mucosal and systemic SARS-CoV-2 specific antibodies, to comprehensively characterise SARS-CoV-2 infection and transmission in households. Methods Between March and September 2020, we obtained samples from 92 participants in 26 households in Melbourne, Australia, in a 4-week period following onset of infection with ancestral SARS-CoV-2 variants. Results The secondary attack rate was 36% (24/66) when using nasopharyngeal swab (NPS) PCR positivity alone. However, when respiratory and non-respiratory samples were combined with antibody responses in blood and saliva, the secondary attack rate was 76% (50/66). SARS-CoV-2 viral load of the index case and household isolation measures were key factors that determine secondary transmission. In 27% (7/26) of households, all family members tested positive by NPS for SARS-CoV-2 and were characterised by lower respiratory Ct-values than low transmission families (Median 22.62 vs 32.91; IQR 17.06 to 28.67 vs 30.37 to 34.24). High transmission families were associated with enhanced plasma antibody responses to multiple SARS-CoV-2 antigens and the presence of neutralising antibodies. Three distinguishing saliva SARS-CoV-2 antibody features were identified according to age (IgA1 to Spike 1, IgA1 to nucleocapsid protein (NP), suggesting that adults and children generate distinct mucosal antibody responses during the acute phase of infection. Conclusion Utilising respiratory and non-respiratory PCR testing, along with measurement of SARS-CoV-2 specific local and systemic antibodies, provides a more accurate assessment of infection within households and highlights some of the immunological differences in response between children and adults. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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