| Autor: |
Anna De Marco, Amalia De Santo Virzo, Edgardo Filippone, Pasquale Chiaiese, Antonio Di Matteo, Gianluca Ruotolo |
| Přispěvatelé: |
Chiaiese, Pasquale, G., Ruotolo, DI MATTEO, Antonio, A., Virzo De Santo, DE MARCO, Anna, Filippone, Edgardo |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Industrial Crops and Products. 34:1072-1078 |
| ISSN: |
0926-6690 |
| Popis: |
In order to apply state-of-the-art molecular breeding techniques in fibre crop it is necessary to have a good knowledge of major polymer biosynthesis gene sequences and their expression pattern. Polymerase chain reaction was employed to isolate sequences of the major genes for lignin and cellulose biosynthesis in a kenaf cultivar. CeSA, 4cl, c4h, cad, and ccr gene primers were designed according to their conservative regions; partial sequences of lignin and cellulose biosynthesis genes were obtained. One actin II gene sequence was also isolated from the kenaf genome as a housekeeping gene to be employed in qPCR analysis. Expression levels of genes c4h, cad and CeSA in bark and core from plants harvested at three different growth stages were evaluated. Using qPCR analyses it was found that the expression levels of the two biosynthesis lignin genes in bark tissues increased during plant growth, while a negative trend was recorded in core tissues. In both bark and core, the quantity of lignin was positively correlated to plant growth while cellulose content decreased. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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