Immunocytochemical localization of granulin-1 to mononuclear phagocytic cells of the teleost fish Cyprinus carpio and Carassius auratus
| Autor: | Daniel Belcourt, Yuji Okawara, Hugh P.J. Bennett, James N. Fryer |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Gill
Carps Immunology Acid Phosphatase Molecular Sequence Data Radioimmunoassay Spleen Kidney Cyprinus Goldfish medicine Immunology and Allergy Macrophage Animals Frozen Sections Amino Acid Sequence Carp Conserved Sequence Granulins Head Kidney Phagocytes Paraffin Embedding biology Sequence Homology Amino Acid Immune Sera Proteins Cell Biology biology.organism_classification beta-Galactosidase Molecular biology Immunohistochemistry medicine.anatomical_structure Polyclonal antibodies biology.protein Intercellular Signaling Peptides and Proteins sense organs |
| Zdroj: | Journal of leukocyte biology. 57(1) |
| ISSN: | 0741-5400 |
| Popis: | A new class of low-molecular-weight cysteine-rich regulatory growth factors, designated granulins, has been isolated from hematopoietic tissues of a teleost fish (Cyprinus carpio) and structurally characterized. Granulin-1, the predominant form found in carp spleen, was used to raise polyclonal antibodies in rabbits and to establish a radioimmunoassay. This permitted preliminary tissue distribution studies of granulin-1 to be undertaken in carp (Cyprinus carpio) and goldfish (Carassius auratus). Granulin-1 immunoreactivity was found in the melanomacrophage centers of the spleen and head kidney. Carp tissues anatomically involved in the first line of defense against infection, including skin, gills, gut, and also heart, showed intense granulin-1 immunoreactive staining within presumptive macrophage cells. Granulin-1 immunoreactive macrophages prepared from goldfish spleen and head kidney adhered to glass slides, actively phagocytosed carbon particles, and contained granulin-1 immunoreactivity as well as abundant endogenous peroxidase activity. This study demonstrates that granulin-1 is synthesized and stored in macrophages/monocytes of spleen, head kidney, and peripheral tissues of teleost fish. J. Lcukoc. Biol. 57: 94–100; 1995. |
| Databáze: | OpenAIRE |
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