Translocation Behaviors of Synthetic Polyelectrolytes through Alpha-Hemolysin (α-HL) and Mycobacterium smegmatis Porin A (MspA) Nanopores

Autor:	Xiaoqin Wang, Kaden C. Stevens, Jeffrey M. Ting, Alexander E. Marras, Gelareh Rezvan, Xiaojun Wei, Nader Taheri-Qazvini, Matthew V. Tirrell, Chang Liu
Rok vydání:	2022
Předmět:	Renewable Energy Sustainability and the Environment Materials Chemistry Electrochemistry Condensed Matter Physics Article Surfaces Coatings and Films Electronic Optical and Magnetic Materials
Zdroj:	J Electrochem Soc
ISSN:	1945-7111 0013-4651
DOI:	10.1149/1945-7111/ac6c55
Popis:	DNAs have been used as probes for nanopore sensing of noncharged biomacromolecules due to its negative phosphate backbone. Inspired by this, we explored the potential of diblock synthetic polyelectrolytes as more flexible and inexpensive nanopore sensing probes by investigating translocation behaviors of PEO-b-PSS and PEO-b-PVBTMA through commonly used alpha-hemolysin (α-HL) and Mycobacterium smegmatis porin A (MspA) nanopores. Translocation recordings in different configurations of pore orientation and testing voltage indicated efficient PEO-b-PSS translocations through α-HL and PEO-b-PVBTMA translocations through MspA. This work provides insight into synthetic polyelectrolyte-based probes to expand probe selection and flexibility for nanopore sensing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3344a0a397379d8931830bce00dd5337 https://doi.org/10.1149/1945-7111/ac6c55 Zobrazit plný text záznamu