Transcriptomic analysis of zebrafish (Danio rerio) embryos to assess integrated biotoxicity of Xitiaoxi River waters
| Autor: | Haiyan Yu, Wei Zhang, Tianyi Chu, Zheng Wei, Shengli Zhou, Wenjun Gui, Shuying Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pollution China Embryo Nonmammalian Health Toxicology and Mutagenesis media_common.quotation_subject Drainage basin 010501 environmental sciences Toxicology 01 natural sciences 03 medical and health sciences Rivers Water Quality Environmental monitoring Animals RNA Messenger Zebrafish 0105 earth and related environmental sciences media_common Pollutant geography geography.geographical_feature_category Gene Expression Profiling Aquatic animal General Medicine Acute toxicity 030104 developmental biology Environmental chemistry Environmental science Water quality Surface water Water Pollutants Chemical Environmental Monitoring |
| Zdroj: | Environmental Pollution. 242:42-53 |
| ISSN: | 0269-7491 |
| DOI: | 10.1016/j.envpol.2018.06.060 |
| Popis: | Assessing the toxicity posed by mixtures of unknown chemicals to aquatic organisms is challenging. In this study, water samples from six cross-sections along the Xitiaoxi River Basin (XRB) were monthly or bimonthly collected in 2014. The year-period physiochemical parameters as well as one-month-water sample based acute biotoxicity tests showed that the river water quality of the year was generally in a good status. High performance liquid chromatography (HPLC) screening based on one-month-water samples suggested that the organic pollutants might be non-to-moderately-polar chemicals in very low concentrations. One-month-water sample based RNA-seq was performed to measure the mRNA differential expression profile of zebrafish larvae to furtherly explore the potential bioeffect and the spatial water quality change of the river. Result indicated that the number of deferentially expressed genes (DEGs) tended to increase along the downstream direction of the river. Gene ontology (GO) enrichment analysis implied that the key pollutants might mainly be the function disruptors of biological processes. Principle components analysis (PCA) combining with transcripts and one-month-water sample based physiochemical parameters indicated that the pollution might be similar at TP, DP and CTB sites while pollution homology existed on some extent between YBQ and JW sites. Although the water quality of the river had a complex time-space alternation during the year, and the one-month-data based RNA-seq could not reflex the whole year-water quality of a watershed, the gene expression profile via RNA-seq provided an alternative way for assessing integrated biotoxicity of surface water, and it was relatively fit for early-warning of water quality of a watershed with unobservable acute toxicity. However, the identification of detail toxicants and the links between DEGs and pollution level as well as physiological-biochemical toxicity needed further investigation. |
| Databáze: | OpenAIRE |
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