Stomatin-like protein 2 is overexpressed in epithelial ovarian cancer and predicts poor patient survival
| Autor: | Fei Sun, Xiao-Jing Wang, Wen Ding, Ze-Biao Ma, Yan-Fang Li, Jie-Hua He |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty genetic structures endocrine system diseases Gene Expression Kaplan-Meier Estimate Carcinoma Ovarian Epithelial Surgical oncology Cell Line Tumor Gene expression Genetics Carcinoma Biomarkers Tumor Medicine Humans Neoplasms Glandular and Epithelial RNA Messenger Neoplasm Metastasis Aged Neoplasm Staging Ovarian Neoplasms Messenger RNA business.industry Cancer Membrane Proteins Reproducibility of Results Biomarker Blood Proteins Epithelial ovarian cancer Middle Aged medicine.disease Prognosis female genital diseases and pregnancy complications eye diseases Tumor Burden SLP-2 Oncology Case-Control Studies Cancer research Immunohistochemistry Female sense organs Stem cell Neoplasm Grading business Stomatin Research Article |
| Zdroj: | BMC Cancer |
| ISSN: | 1471-2407 |
| Popis: | Background Stomatin-like protein 2 (SLP-2, also known as STOML2) is a stomatin homologue of uncertain function. SLP-2 overexpression has been suggested to be associated with cancer progression, resulting in adverse clinical outcomes in patients. Our study aim to investigate SLP-2 expression in epithelial ovarian cancer cells and its correlation with patient survival. Methods SLP-2 mRNA and protein expression levels were analysed in five epithelial ovarian cancer cell lines and normal ovarian epithelial cells using real-time PCR and western blotting analysis. SLP-2 expression was investigated in eight matched-pair samples of epithelial ovarian cancer and adjacent noncancerous tissues from the same patients. Using immunohistochemistry, we examined the protein expression of paraffin-embedded specimens from 140 patients with epithelial ovarian cancer, 20 cases with borderline ovarian tumours, 20 cases with benign ovarian tumours, and 20 cases with normal ovarian tissues. Statistical analyses were applied to evaluate the clinicopathological significance of SLP-2 expression. Results SLP-2 mRNA and protein expression levels were significantly up-regulated in epithelial ovarian cancer cell lines and cancer tissues compared with normal ovarian epithelial cells and adjacent noncancerous ovarian tissues. Immunohistochemistry analysis revealed that the relative overexpression of SLP-2 was detected in 73.6 % (103/140) of the epithelial ovarian cancer specimens, 45.0 % (9/20) of the borderline ovarian specimens, 30.0 % (6/20) of the benign ovarian specimens and none of the normal ovarian specimens. SLP-2 protein expression in epithelial ovarian cancer was significantly correlated with the tumour stage (P |
| Databáze: | OpenAIRE |
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