Management of Hepatitis E Virus (HEV) Zoonotic Transmission: Protection of Rabbits against HEV Challenge following Immunization with HEV 239 Vaccine

Autor: Feng min Lu, Peng Liu, Yulin Zhang, Jian Han, Ling Wang, Jun ke Xia, Ren jie Du, Hui Zhuang, Lin Liu
Rok vydání: 2014
Předmět:
viruses
lcsh:Medicine
Antibodies
Viral
medicine.disease_cause
Hepatitis
Feces
Hepatitis E virus
Zoonoses
Emerging Viral Diseases
lcsh:Science
Small Animals
Vaccines
Synthetic
Vaccines
education.field_of_study
Multidisciplinary
Zoonotic Diseases
Viral Vaccine
Vaccination
virus diseases
Antivirals
Hepatitis E
Immunizations
Specific Pathogen-Free Organisms
Virus Shedding
Veterinary Diseases
Medicine
Infectious diseases
Rabbits
Viral hepatitis
Research Article
Viral Hepatitis Vaccines
Animal Types
Population
Viremia
Viral diseases
Biology
Microbiology
Virology
Microbial Control
medicine
Animals
Hepatitis Antibodies
Viral shedding
education
lcsh:R
Immunity
Viral Vaccines
medicine.disease
digestive system diseases
Emerging Infectious Diseases
Immunology
lcsh:Q
Immunization
Clinical Immunology
Veterinary Science
Viral Transmission and Infection
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 1, p e87600 (2014)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0087600
Popis: Hepatitis E virus (HEV) constitutes a significant health burden worldwide, with an estimated approximately 33% of the world's population exposed to the pathogen. The recent licensed HEV 239 vaccine in China showed excellent protective efficacy against HEV of genotypes 1 and 4 in the general population and pregnant women. Because hepatitis E is a zoonosis, it is also necessary to ascertain whether this vaccine can serve to manage animal sources of human HEV infection. To test the efficacy of the HEV 239 vaccine in protecting animal reservoirs of HEV against HEV infection, twelve specific-pathogen-free (SPF) rabbits were divided randomly into two groups of 6 animals and inoculated intramuscularly with HEV 239 and placebo (PBS). All animals were challenged intravenously with swine HEV of genotype 4 or rabbit HEV seven weeks after the initial immunization. The course of infection was monitored for 10 weeks by serum ALT levels, duration of viremia and fecal virus excretion and HEV antibody responses. All rabbits immunized with HEV 239 developed high titers of anti-HEV and no signs of HEV infection were observed throughout the experiment, while rabbits inoculated with PBS developed viral hepatitis following challenge, with liver enzyme elevations, viremia, and fecal virus shedding. Our data indicated that the HEV 239 vaccine is highly immunogenic for rabbits and that it can completely protect rabbits against homologous and heterologous HEV infections. These findings could facilitate the prevention of food-borne sporadic HEV infection in both developing and industrialized countries.
Databáze: OpenAIRE
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