Comparison of the antiviral potentials among the pseudorabies-resistant transgenes encoding different soluble forms of porcine nectin-1 in transgenic mice
| Autor: | Yuki Watanabe, Pierre Cherel, Etsuro Ono, Keiko Amagai, Masami Morimatsu, Kyoko Shinya, Yukiko Tomioka |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Genetically modified mouse
biology Swine Recombinant Fusion Proteins Transgene Nectins Pseudorabies Mice Transgenic biology.organism_classification Antiviral Agents Herpesvirus 1 Suid Polymerase Chain Reaction Fusion protein Virology Virus Immunoglobulin Fc Fragments Mice Chimera (genetics) Ectodomain biology.protein Animals Antibody Cell Adhesion Molecules |
| Zdroj: | Journal of General Virology. 88:2636-2641 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.83080-0 |
| Popis: | Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D by the first immunoglobulin (Ig)-like domain. The possibility of making animals resistant to pseudorabies virus (PRV) infection has been investigated by generating transgenic mice expressing soluble forms of porcine nectin-1. Previously, transgenic mice were generated that expressed a fusion protein made of the entire ectodomain of nectin-1 fused to the Fc portion of human IgG, or the first Ig-like domain fused to the Fc portion of porcine IgG. Here, the contribution of the second and third Ig-like domains of nectin-1 was analysed by generating transgenic mice expressing the entire ectodomain of nectin-1 fused to the porcine Fc portion. Transgenic mice expressing each of three different fusion proteins were challenged with PRV for comparison of their resistance. Altogether, mice transgenic for a chimera that carried the entire ectodomain were more resistant than those transgenic for a chimera that carried the first Ig-like domain. |
| Databáze: | OpenAIRE |
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