Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells
| Autor: | Michael P. Bolognesi, Virginia B. Kraus, M.-F. Hsueh, Samuel S. Wellman |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Lipopolysaccharide Neutrophils THP-1 Cells T-Lymphocytes Interleukin-1beta Biomedical Engineering Inflammation Naproxen Sodium Pharmacology Article Dinoprostone Monocytes 03 medical and health sciences chemistry.chemical_compound Naproxen 0302 clinical medicine Immune system Rheumatology Synovial Fluid medicine Humans Synovial fluid Orthopedics and Sports Medicine Interleukin 8 Hyaluronic Acid Prostaglandin E2 030203 arthritis & rheumatology Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Anti-Inflammatory Agents Non-Steroidal Interleukin-8 NF-kappa B Osteoarthritis Knee Flow Cytometry 030104 developmental biology chemistry Cell culture Cytokines medicine.symptom medicine.drug |
| Zdroj: | Osteoarthritis Cartilage |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2020.01.013 |
| Popis: | Summary Objective To evaluate the anti-inflammatory effects of clinically relevant naproxen sodium (Nx) concentrations on human monocyte-derived macrophages in a controlled in vitro system and human primary synovial fluid (SF) cells. Design Using phorbol 12-myristate 13-acetate, THP-1 human monocytic cells were differentiated into mature monocyte-derived macrophages in vitro then treated with Nx pre- or post-activating an inflammatory response with lipopolysaccharide (LPS) and hyaluronan (HA) fragments (n = 8/group). Cell culture supernatants were assessed for NF-κB activity and prostaglandin E2 (PGE2), indicating cyclooxygenase enzyme activity. Under Duke IRB approval, primary human SF cells were collected at the time of knee joint replacement (n = 19 individuals) for osteoarthritis (OA), and cultured with LPS, HA and Nx; SF cells were characterized by polychromatic flow cytometry for cell surface markers and intracellular cytokines. Result Compared to placebo treatment of THP-1 cells, low dose Nx (corresponding 27.5–440 mg/L orally) added both pre- and post-activation with LPS/HA, significantly reduced NF-κB activity and PGE2: mean reduction to 73%, 61%, 17% and 10% of placebo, respectively. LPS/HA treatment of primary OA SF cells significantly increased the number of IL-1β producing primary monocytes and macrophages, and by 24 h the overall production of secreted cytokines (IL-1β, IL-6, IL8, and TNF-α). Low dose Nx reduced the percentage of IL-1β producing primary monocytes and macrophages. Conclusion LPS/HA induced inflammation of THP-1 monocytic and primary human SF cells. Low dose Nx both prevented and reduced inflammatory responses of a human monocytic cell line and reduced IL-1β production by primary human SF monocytes and macrophages. |
| Databáze: | OpenAIRE |
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