Inhibitory effects of non-steroidal anti-inflammatory drugs on superoxide generation
| Autor: | Yasukatsu Yuda, Kazuei Igarashi, Koukichi Suzuki, Junko Tanaka, Tetsuo Satoh |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Ketoprofen Neutrophils Sodium chemistry.chemical_element Pharmacology chemistry.chemical_compound Superoxides Drug Discovery medicine Animals NADH NADPH Oxidoreductases Xanthine oxidase Cells Cultured Oxidase test NADPH oxidase biology Superoxide Anti-Inflammatory Agents Non-Steroidal Zymosan NADPH Oxidases Rats Inbred Strains General Chemistry General Medicine Diclofenac Sodium Rats Biochemistry chemistry biology.protein medicine.drug |
| Zdroj: | Chemical and Pharmaceutical Bulletin. 39:1075-1077 |
| ISSN: | 1347-5223 0009-2363 |
| DOI: | 10.1248/cpb.39.1075 |
| Popis: | Effects of non-steroidal anti-inflammatory drugs (NSAID: amfenac sodium, diclofenac sodium, indomethacin and ketoprofen) on the generation of superoxide anion (O2-) by isolated rat polymorphonuclear leukocytes (PMN) were studied spectrophotometrically using cytochrome c. The effects of these drugs were also studied on O2- production by the xanthine-xanthine oxidase and reduced nicotinamide adenine dinucleotide phosphate (NADPH)-NADPH oxidase systems. Amfenac sodium, at 0.1 mM, inhibited significantly O2- generation in rat PMN induced by opsonized zymosan. At 0.5 mM, diclofenac sodium and indomethacin inhibited the O2- generation in rat PMN. All of the above drugs slightly inhibited O2- production by the xanthine-xanthine oxidase system. On the other hand, O2- production by the NADPH-NADPH oxidase system was significantly inhibited by the addition of amfenac sodium, ketoprofen or indomethacin. These results suggest that non-steroidal anti-inflammatory drugs do not work as an O2- scavenger and block O2- production by the NADPH-NADPH oxidase system of rat PMN. It is concluded that amfenac sodium and the other drugs are able to inhibit granulocyte O2- production by blocking the activation of NADPH-oxidase. |
| Databáze: | OpenAIRE |
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