Assessment of potential SARS-CoV-2 virus integration into human genome reveals no significant impact on RT-qPCR COVID-19 testing
| Autor: | Dylan Law, Erica M. Briggs, Paolo Mita, Katharine K. Nelson, William Ward, Sol Rey, Michael Bois, Henry H. Lee, Nili Ostrov, Jon M Laurent |
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| Rok vydání: | 2021 |
| Předmět: |
Multidisciplinary
Genome Human SARS-CoV-2 Virus Integration viruses fungi Zhàng COVID-19 Retrotransposon Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Virology Genome COVID-19 Testing Real-time polymerase chain reaction Pandemic medicine Humans RNA Viral Human genome Letters Coronavirus |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.2113065118 |
| Popis: | Severe acute respiratory coronavirus 2 (SARS-CoV-2) pandemic presents new scientific and scale-up challenges for diagnostic capabilities worldwide. The gold standard diagnostic for SARS-CoV-2 infection is an RT-qPCR which targets the SARS-CoV-2 genome, an assay that has now been performed on millions of patient specimens worldwide regardless of symptomatic status. Zhang et al. (1) suggest the possibility that the SARS-CoV-2 N gene could integrate into host cell DNA through the action of the LINE1 retrotransposon, a mobile element potentially active in human somatic cells, thereby calling into question the veracity of N gene−based RT-qPCR for detection of SARS-CoV-2 infection. Multiple studies and a peer-reviewed work (2) have challenged Zhang et al.’s conclusions, attributing the observed viral/human chimeric sequencing reads to artifactual processes happening during library preparation. Moreover, many are the caveats of Zhang et al.’s hypothesis of SARS-COV-2 … [↵][1] 1To whom correspondence may be addressed. Email: paolo.mita{at}reopenlabs.com. [1]: #xref-corresp-1-1 |
| Databáze: | OpenAIRE |
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