Potential involvement of a constitutive heat shock element binding factor in the regulation of chemical stress-inducedhsp70 gene expression
| Autor: | Peter M. Corry, Yong J. Lee, Richard Y. Liu |
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| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic Arsenites Blotting Western Molecular Sequence Data Clinical Biochemistry Down-Regulation CHO Cells Regulatory Sequences Nucleic Acid Biology chemistry.chemical_compound Cadmium Chloride Chlorides Stress Physiological Cricetinae Heat shock protein Animals HSP70 Heat-Shock Proteins RNA Messenger Heat shock Molecular Biology Arsenite Regulation of gene expression Base Sequence Chinese hamster ovary cell Antibodies Monoclonal Cell Biology General Medicine Blotting Northern Sodium Compounds Molecular biology Salicylates Hsp70 Cell biology DNA-Binding Proteins Heat shock factor Gene Expression Regulation chemistry Cell culture DNA Probes Cadmium |
| Zdroj: | Molecular and Cellular Biochemistry. 144:27-34 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | It was reported that chemical stresses such as arsenite, cadmium or salicylate fail to induce synthesis of the inducible form of HSP70 (HSP70i). We report here that exposure of cells to higher doses of these chemical treatments induced significant synthesis of HSP70i in CHO cells as well as other cell lines. The synthesis of HSP70i is primarily regulated at the transcriptional level. Although all tested chemical treatments induced heat shock factor (HSF) binding to the heat shock element (HSE), HSP70i synthesis appears to be regulated by an alternative factor (CHBF) which constitutively binds to the HSE at 37 degrees C. The treatments, which dissociate the HSE-CHBF complex, induced significant HSP70i synthesis. The treatments, which failed to induce HSP70i synthesis, still activated HSF binding to HSE but the HSE-CHBF complex remained as that of untreated control cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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