Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non‑small cell lung cancer xenografts
| Autor: | Yanjie Lu, Long Chen, Qian Xu, Qin Zhang, Jianping Wang, Xuerong Zhao, Lei Liu, Yanzhen Zuo, Yali Lian, Lei Zhang, Jia Du |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cancer Research Lung Neoplasms PAa Cell Apoptosis Tripartite Motif-Containing Protein 28 Biochemistry Mice 0302 clinical medicine RNA interference Carcinoma Non-Small-Cell Lung Gene Knockdown Techniques RNA Small Interfering Gene knockdown Apoptosis Regulator Cell Cycle Articles Middle Aged Cell cycle nude mice Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Molecular Medicine ELISA Female RNA Interference Adult Mice Nude Biology 03 medical and health sciences Cell Line Tumor Genetics medicine Animals Humans Molecular Biology non-small cell lung cancer Aged Autoantibodies Cell Proliferation Cell growth TRIM28 small interfering RNA 030104 developmental biology Case-Control Studies Cancer research Apoptosis Regulatory Proteins |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.7955 |
| Popis: | TRIM28 is a well‑known transcriptional co‑repressor of Kruppel‑associated box zinc finger proteins. The authors previously demonstrated that TRIM28 small interfering (si)RNA decreases cell proliferation and inhibits cell cycle progression in non‑small cell lung cancer (NSCLC) cell lines. The present study further demonstrated that the stable silencing of TRIM28 expression by a specific siRNA lentivirus vector significantly inhibited the growth and exerted obvious anti‑tumor effects in nude mice. The results of the terminal deoxynucleotidyl transferase‑mediated deoxyuridine triphosphate nick‑end labeling assay indicated that TRIM28 knockdown increased apoptosis. Furthermore, TRIM28 knockdown decreased the expression of B cell lymphoma (Bcl)‑2 and increased the expression of Bcl‑2 associated X, apoptosis regulator and p53 at the gene and protein levels. Auto‑antibodies to TRIM28 were present in 12.32% of the sera of the patients with NSCLC. The results suggest that TRIM28 knockdown may be effective against NSCLC, and TRIM28 antibodies have the potential to act as novel diagnostic and therapeutic tools. |
| Databáze: | OpenAIRE |
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