Down-regulation of steroidogenic cytochrome P450XVII in cryptorchid rat testes
Autor: | Rediger J, Staib W, Kühn-Velten N |
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Rok vydání: | 1990 |
Předmět: |
Male
endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism Clinical Biochemistry Down-Regulation Biology Testicle Biochemistry Endocrinology Downregulation and upregulation Cytochrome P-450 Enzyme System In vivo Internal medicine Microsomes Cryptorchidism Testis medicine Animals Involution (medicine) Incubation Leydig cell urogenital system Biochemistry (medical) Temperature Cytochrome P450 Leydig Cells Rats Inbred Strains General Medicine Rats medicine.anatomical_structure biology.protein Microsome |
Zdroj: | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 22(10) |
ISSN: | 0018-5043 |
Popis: | The objective of the present study was to investigate the regulation of a key component of testicular androgen biosynthesis, i.e. the cytochrome P450XVII of the steroid-17 alpha-monooxygenase/C17,20-lyase, after surgical induction of bilateral cryptorchidism in vivo. Seven days after induction of cryptorchidism, P450XVII concentrations are diminished (as compared to sham-operated controls) by 64% in isolated purified Leydig cells but only by 44% in the total Leydig cell compartment of the testis, since the Leydig cell yield from cryptorchid testes is by 53% higher than that from control testes. Using microsomal suspensions prepared from testicular homogenates, P450XVII content per testis equivalent is found to be decreased by 36% seven days after incubation of cryptorchidism, whereas the P450XVII concentration per gram testis is not changed due to testicular involution. Fourteen days after induction of cryptorchidism, the induction of the Leydig cell system appears to superimpose on the down-regulation of P450XVII. The study demonstrates both a strong sensitivity of P450XVII to short-term elevation of testicular temperature and a differentiation between effects of cryptorchidism on total testicular content and specific cellular and subcellular concentration of this steroidogenic protein. |
Databáze: | OpenAIRE |
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