Growth and enterotoxin production of Bacillus cereus in cow, goat, and sheep milk
| Autor: | Šárka Bursová, Žaneta Ševčíková, Pavla Prachařová, B. Janštová, Alena Skočková, Lenka Necidová, Bohdana Janštová |
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| Rok vydání: | 2014 |
| Předmět: |
diarrhoeal enterotoxins
Plate method lcsh:Veterinary medicine General Veterinary biology Inoculation Chemistry fungi raw milk Bacillus cereus pasteurized milk food and beverages Pasteurization Enterotoxin Raw milk biology.organism_classification Food safety law.invention fluids and secretions Cereus law lcsh:SF600-1100 Food science Sheep milk |
| Zdroj: | Acta Veterinaria Brno, Vol 83, Iss 10, Pp 3-8 (2014) |
| ISSN: | 1801-7576 0001-7213 |
| Popis: | The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 °C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 °C (sheep milk) and 22 °C (cow and goat milk). Enterotoxin presence was detected at 15 °C and 22 °C, and with pasteurized cow milk also at 8 °C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type. |
| Databáze: | OpenAIRE |
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