The Application of Magnetic Cell Sorter (MACS) to Detect Fetal Cells in Maternal Peripheral Blood
| Autor: | Akimune Fukushima, Yukari Utsugisawa, Noriko Mizusawa, Teruo Kagabu, Yuko Wada, Saburo Horiuchi |
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| Rok vydání: | 2001 |
| Předmět: |
Electrophoresis
Male Sex Determination Analysis Pathology medicine.medical_specialty Erythroblasts Gestational Age Cell Separation Polymerase Chain Reaction Magnetics Cell sorter Pregnancy Y Chromosome medicine Humans False Positive Reactions Deoxyribonucleases Type II Site-Specific False Negative Reactions Fetus business.industry Obstetrics Infant Newborn Obstetrics and Gynecology Gestational age Nucleated Red Blood Cell Fetal Blood medicine.disease Peripheral blood Gestation Female business |
| Zdroj: | Journal of Obstetrics and Gynaecology Research. 27:155-162 |
| ISSN: | 1447-0756 1341-8076 |
| DOI: | 10.1111/j.1447-0756.2001.tb01240.x |
| Popis: | Objectives: The aim of the present study was to investigate the effectiveness of sorting fetal nucleated red blood cells (FNRBC) from maternal peripheral blood, particularly during early gestation periods, by a combination of specific gravity centrifugation and magnetic cell sorter (MACS). Methods: Without prior knowledge of the gender of the fetus, we determined gender by analyzing a Y-chromosome specific sequence by nested-PCR, using 10 ml of the peripheral blood of healthy primigravida women at different stages of gestation (first trimester: n = 17, second trimester: n = 13, and third trimester: n = 19). The results of this prenatal sex determination were compared to the sex of newborns. Results: The specificity, sensitivity, positive predictive value (PPV) and negative predictive value (NPV) of the present method during the first trimester were 100, 81.8, 100, and 75%, respectively; during the second trimester, 80, 50, 80, and 50%, respectively; and during the third trimester, 25, 63.6, 53.8, and 33.3%, respectively. Conclusion: The results show that this prenatal sex determination method has a highly accurate diagnostic rate during the first trimester, suggesting that it could be developed as a practical, non-invasive prenatal diagnostic technique for use during early gestation periods. |
| Databáze: | OpenAIRE |
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