Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spines
| Autor: | David Mazaud, Alexis Bemelmans, Lilia Kushnireva, David Holcman, Kanishka Basnayake, Nathalie Rouach, Eduard Korkotian |
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| Přispěvatelé: | Basnayake, Kanishka [0000-0001-8772-1158], Kushnireva, Lilia [0000-0002-6107-5052], Bemelmans, Alexis [0000-0001-7605-5225], Rouach, Nathalie [0000-0002-5574-888X], Korkotian, Eduard [0000-0001-5515-3387], Holcman, David [0000-0001-9854-5014], Apollo - University of Cambridge Repository, Center for Interdisciplinary Research in Biology, College de France, CNRS, INSERM, PSL Research University, 75231 Paris, France. (CIRB) |
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
[PHYS]Physics [physics] 0303 health sciences endocrine system Multidisciplinary Dendritic spine 3208 Medical Physiology Chemistry [SDV]Life Sciences [q-bio] FOS: Clinical medicine Calcium diffusion Neurosciences food and beverages 32 Biomedical and Clinical Sciences musculoskeletal system Spine apparatus [STAT]Statistics [stat] 03 medical and health sciences 0302 clinical medicine Biophysics 030217 neurology & neurosurgery ComputingMilieux_MISCELLANEOUS 030304 developmental biology 31 Biological Sciences |
| Zdroj: | Science Advances Science Advances, American Association for the Advancement of Science (AAAS), 2021, 7 (38), ⟨10.1126/sciadv.abh1376⟩ |
| ISSN: | 2375-2548 |
| DOI: | 10.17863/cam.77003 |
| Popis: | Dendritic spines are critical components of neuronal synapses as they receive and transform synaptic inputs into a succession of calcium-regulated biochemical events. The spine apparatus (SA), an extension of smooth endoplasmic reticulum, regulates slow and fast calcium dynamics in spines. Calcium release events deplete SA calcium ion reservoir rapidly, yet the next cycle of signaling requires its replenishment. How spines achieve this replenishment without triggering calcium release remains unclear. Using computational modeling, calcium and STED superresolution imaging, we show that the SA replenishment involves the store-operated calcium entry pathway during spontaneous calcium transients. We identified two main conditions for SA replenishment without depletion: a small amplitude and a slow timescale for calcium influx, and a close proximity between SA and plasma membranes. Thereby, spine’s nanoscale organization separates SA replenishment from depletion. We further conclude that spine’s receptor organization also determines the calcium dynamics during the induction of long-term synaptic changes. |
| Databáze: | OpenAIRE |
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