Immunomodulatory effects of bone marrow mesenchymal stem cells overexpressing heme oxygenase-1: Protective effects on acute rejection following reduced-size liver transplantation in a rat model
| Autor: | Hong-Li Song, Wei-Ping Zheng, Bin Wu, Mingli Yin, Zhong-yang Shen, Tao Liu, Bo-Ya Zhang, Yang Yang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Graft Rejection
Male 0301 basic medicine medicine.medical_treatment Immunology Mesenchymal Stem Cell Transplantation T-Lymphocytes Regulatory Proinflammatory cytokine Natural killer cell Immunomodulation 03 medical and health sciences Animals Transplantation Homologous Medicine Transgenes IL-2 receptor Th1-Th2 Balance Cells Cultured Innate immune system business.industry Mesenchymal stem cell Mesenchymal Stem Cells Liver Transplantation Rats Heme oxygenase 030104 developmental biology Cytokine medicine.anatomical_structure Rats Inbred Lew Acute Disease Th17 Cells Transplantation Tolerance business Heme Oxygenase-1 Ex vivo |
| Zdroj: | Cellular Immunology. 313:10-24 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/j.cellimm.2016.12.006 |
| Popis: | Here we explore the T-lymphocyte suppressive and immunomodulatory effects of bone marrow mesenchymal stem cells (BMMSCs) overexpressing heme oxygenase-1 (HO-1) on acute rejection following reduced-size liver transplantation (RLT) in a rat model. The proliferation activity, cell cycle progression, secretion of proinflammatory cytokines, expression of CD25 and CD71 in lymphocytes, and activity of NK cells were found to be significantly lowered, and the proportion of regulatory T cells (Tregs) was found to be increased relative to BMMSCs when Adv-HO-1/BMMSCs were co-cultured with Con A ex vivo; secretion of anti-inflammatory cytokines was significantly higher. When treated with saline, BMMSCs or Adv-HO-1/BMMSCs, post-transplantation rats receiving Adv-HO-1/BMMSCs showed better median survival time, lower rejection activity index, higher anti-inflammatory cytokine levels, lower proinflammatory cytokine levels, more peripheral Tregs, and lower natural killer cell viability. These results suggest that HO-1 enhanced and prolonged the effects of BMMSCs on acute rejection following RLT, with immunomodulatory effects in which adaptive and innate immunity, as well as paracrine signaling, may play important roles. |
| Databáze: | OpenAIRE |
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