Isolation and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants
| Autor: | Laurent G. Deluc, François Barrieu, Rim Mzid, Chloé Marchive, Farid Regad, Adrien Gauthier, Saïd Hamdi, Virginie Lauvergeat, Bernard Cailleteau, Marie-France Corio-Costet, Julien Pirrello |
|---|---|
| Přispěvatelé: | Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Unité mixte de recherche santé végétale, Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), ALTADIS |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
0106 biological sciences
Génomique et Biotechnologie des Fruits Physiology Transgene salicylic acid [SDV]Life Sciences [q-bio] Amino Acid Motifs Molecular Sequence Data WRKY transcription factor Plant Science Genetically modified crops Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound plant resistance to pathogens Gene Expression Regulation Plant Complementary DNA Botany Gene expression Tobacco [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Vitis Cloning Molecular Pathogen 030304 developmental biology Plant Proteins 2. Zero hunger Genetics chemistry.chemical_classification 0303 health sciences Base Sequence Fungi food and beverages Plants Genetically Modified WRKY protein domain Immunity Innate Amino acid [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy chemistry Salicylic acid 010606 plant biology & botany Transcription Factors |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2007, 58 (8), pp.1999-2010. ⟨10.1093/jxb/erm062⟩ |
| ISSN: | 0022-0957 1460-2431 |
| DOI: | 10.1093/jxb/erm062⟩ |
| Popis: | International audience; Pathogen attack represents a major problem for viticulture and for agriculture in general. At present, the use of phytochemicals is more and more restrictive, and therefore it is becoming essential to control disease by having a thorough knowledge of resistance mechanisms. The present work focused on the trans-regulatory proteins potentially involved in the control of the plant defence response, the WRKY proteins. A full-length cDNA, designated VvWRKY1, was isolated from a grape berry library (Vitis vinifera L. cv. Cabernet Sauvignon). It encodes a polypeptide of 151 amino acids whose structure is characteristic of group IIc WRKY proteins. VvWRKY1 gene expression in grape is regulated in a developmental manner in berries and leaves and by various signal molecules involved in defence such as salicylic acid, ethylene, and hydrogen peroxide. Biochemical analysis indicates that VvWRKY1 specifically interacts with the W-box in various nucleotidic contexts. Functional analysis of VvWRKY1 was performed by overexpression in tobacco, and transgenic plants exhibited reduced susceptibility to various fungi but not to viruses. These results are consistent with a possible role for VvWRKY1 in grapevine defence against fungal pathogens. |
| Databáze: | OpenAIRE |
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