Induction of lung glutathione and glutamylcysteine ligase by 1,4-phenylenebis(methylene)selenocyanate and its glutathione conjugate: role of nuclear factor-erythroid 2-related factor 2
| Autor: | Shantu Amin, Karam El-Bayoumy, Dhimant Desai, John P. Richie, Sans W. Emmert, Cesar Aliaga, Yuan-Wan Sun, Arunangshu Das |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Small interfering RNA NF-E2-Related Factor 2 Glutamate-Cysteine Ligase Biochemistry Article Cell Line chemistry.chemical_compound Mice Physiology (medical) Organoselenium Compounds Animals Humans Inducer Luciferase Antioxidant Response Elements RNA Small Interfering Lung Mice Knockout Kinase GCLM Glutathione Hep G2 Cells respiratory system Molecular biology Rats GCLC chemistry Liver RNA Interference |
| Zdroj: | Free radical biologymedicine. 52(10) |
| ISSN: | 1873-4596 |
| Popis: | The synthetic organoselenium agent 1,4-phenylenebis(methylene)selenocyanate (p-XSC) and its glutathione (GSH) conjugate (p-XSeSG) are potent chemopreventive agents in several preclinical models. p-XSC is also an effective inducer of GSH in mouse lung. Our objectives were to test the hypothesis that GSH induction by p-XSC occurs through upregulation of the rate-limiting GSH biosynthetic enzyme glutamylcysteine ligase (GCL), through activation of antioxidant response elements (AREs) in GCL genes via activation of nuclear factor-erythroid 2-related factor 2 (Nrf2). p-XSC feeding (10 ppm Se) increased GSH (230%) and upregulated the catalytic subunit of GCL (GCLc) (55%), extracellular-related kinase (220%), and nuclear Nrf2 (610%) in lung but not liver after 14 days in the rat (P |
| Databáze: | OpenAIRE |
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