The Effect of royal jelly on boar sperm viability and motility during liquid storage for 96 hours
| Autor: | Henrikas Žilinskas, Aiste Iljenkaite, Zoja Mikniene, Agila Dauksiene, Neringa Sutkeviciene, Sigita Kerzienė |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
030219 obstetrics & reproductive medicine food.ingredient General Veterinary Chemistry urogenital system 0402 animal and dairy science Motility 04 agricultural and veterinary sciences 040201 dairy & animal science Andrology 03 medical and health sciences 0302 clinical medicine food Royal jelly Liquid storage Honey porcine semen quality bee products Boar sperm |
| Zdroj: | Acta veterinaria Brno, Brno : University School Of Veterinary Medicine, 2020, vol. 89, no. 1, p. 47-53 |
| ISSN: | 0001-7213 1801-7576 |
| Popis: | The current study was carried out to investigate the protective effects of royal jelly supplementation on sperm motility, viability and pH value during the liquid storage of boar semen at 16 °C and 4 °C, at various periods of time (0, 24, 48, 72 and 96 h). Semen samples were collected from 11 boars, diluted with a long-term extender and supplemented with different concentration of royal jelly (0%, 0.5%, 1% and 2%) at a final concentration of 50 × 106 sperm/ml. In the laboratory, the semen was assessed for sperm morphology, viability (eosin-nigrosin staining), subjective motility and objective sperm motility by sperm class analyzer. In total, 396 tests for sperm viability and motility were performed. The longer storage time and the lower incubation temperature showed lower sperm motility and viability results. The results showed that royal jelly supplementation at 1% concentrations protected the functionality of the sperm plasma membrane during the liquid storage time of 96 h at 16 °C. Sperm subjective and objective motility results in samples stored at 4 °C decreased with higher royal jelly concentrations and longer storage time, and differ significantly from the results in samples stored at 16 °C (P < 0.05). Our data showed that royal jelly supplementation at lower concentrations can improve boar semen motility and viability parameters during liquid storage at 16 °C for 96 h. |
| Databáze: | OpenAIRE |
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