Transient and Persistent Gastric Microbiome: Adherence of Bacteria in Gastric Cancer and Dyspeptic Patient Biopsies after Washing
| Autor: | Malene Roed Spiegelhauer, Laimas Virginijus Jonaitis, Limas Kupčinskas, Thor Bech Johannesen, Juozas Kupcinskas, Leif P. Andersen, Mindaugas Urba, Kurt Fuursted, Tove Havnhøj Frandsen, Jurgita Skieceviciene |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
Atrophic gastritis Peptic Antibiotics gastric microbiota transient persistent culture microbiome sequencing Helicobacter pylori lcsh:Medicine Article Microbiology 03 medical and health sciences 0302 clinical medicine medicine Microbiome 030304 developmental biology 0303 health sciences biology business.industry Stomach lcsh:R Cancer General Medicine biology.organism_classification medicine.disease digestive system diseases medicine.anatomical_structure 030211 gastroenterology & hepatology business Bacteria |
| Zdroj: | Journal of Clinical Medicine Volume 9 Issue 6 Journal of Clinical Medicine, Vol 9, Iss 1882, p 1882 (2020) Journal of Clinical Medicine, Basel : MDPI, 2020, vol. 9, no. 6, 1882, p. 1-23 |
| ISSN: | 2077-0383 |
| DOI: | 10.3390/jcm9061882 |
| Popis: | Helicobacter pylori is a common colonizer of the human stomach, and long-term colonization has been related to development of atrophic gastritis, peptic ulcers and gastric cancer. The increased gastric pH caused by H. pylori colonization, treatment with antibiotics or proton pump inhibitors (PPI) may allow growth of other bacteria. Previous studies have detected non-Helicobacter bacteria in stomach biopsies, but no conclusion has been made of whether these represent a transient contamination or a persistent microbiota. The aim of this study was to evaluate the transient and persistent bacterial communities of gastric biopsies. The washed or unwashed gastric biopsies were investigated by cultivation and microbiota analysis (16S rRNA gene-targeted amplicon sequencing) for the distribution of H. pylori and other non-Helicobacter bacteria. The number of cultured non-Helicobacter bacteria decreased in the washed biopsies, suggesting that they might be a transient contamination. No significant differences in the bacterial diversity were observed in the microbiome analysis between unwashed and washed biopsies. However, the bacterial diversity in biopsies shown H. pylori-positive and H. pylori-negative were significantly different, implying that H. pylori is the major modulator of the gastric microbiome. Further large-scale studies are required to investigate the transient and persistent gastric microbiota. |
| Databáze: | OpenAIRE |
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