Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae
| Autor: | Chawki Bensouici, Artur M. S. Silva, Susana M. Cardoso, Habiba Rechek, Diana C. G. A. Pinto, Noureddine Soltani, Kaouther Hamaidia, Hamza Allal, Tarek Boudiar, Ammar Haouat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
medicine.drug_class medicine.medical_treatment Pharmaceutical Science Organic chemistry antioxidant activity phenolic compounds Warionia saharae Anti-inflammatory Analytical Chemistry chemistry.chemical_compound QD241-441 Galvinoxyl Chromatography detector Drug Discovery medicine Taxifolin GC–MS analysis Physical and Theoretical Chemistry anti-inflammatory activity Scavenging enzyme inhibition UHPLC-DAD-ESI/MS chemistry.chemical_classification biology Traditional medicine molecular docking Asteraceae biology.organism_classification Enzyme chemistry Chemistry (miscellaneous) Molecular Medicine |
| Zdroj: | Molecules Volume 26 Issue 17 Molecules, Vol 26, Iss 5257, p 5257 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26175257 |
| Popis: | Warionia saharae Benth. & Coss. (Asteraceae) is an endemic species of North Africa naturally grown in the southwest of the Algerian Sahara. In the present study, this species’ hydromethanolic leaf extract was investigated for its phenolic profile characterized by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS). Additionally, the chemical composition of W. saharae was analyzed by gas chromatography–mass spectrometry, and its antioxidant potential was assessed through five in vitro tests: DPPH● scavenging activity, ABTS●+ scavenging assay, galvinoxyl scavenging activity, ferric reducing power (FRP), and cupric reducing antioxidant capacity. The UHPLC-DAD-ESI/MS analysis allowed the detection and quantification of 22 compounds, with taxifolin as the dominant compound. The GC–MS analysis allowed the identification of 37 compounds, and the antioxidant activity data indicate that W. saharae extract has a very high capacity to capture radicals due to its richness in compounds with antioxidant capacity. The extract also showed potent α-glucosidase inhibition as well as a good anti-inflammatory activity. However, weak anti-α-amylase and anticholinesterase activities were recorded. Moreover, an in silico docking study was performed to highlight possible interactions between three significant compounds identified in W. saharae extract and α-glucosidase enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|